Summary
Receptor cells in the ear are mechanically excited through displacement of sensory hairs, stereocilia, in relation to a sub-surface platform, the cuticular plate, into which rootlets of the stereocilia insert.
The presence of actin in inner ear sensory organs and receptor cells was established by gel electrophoresis, by labelling with antibodies against actin, and by electron microscopy after decoration with subfragment-1 of Myosin. The latter method was used to determine the functional orientation of actin filaments found to be present in the mechanosensitive region of the receptor cells. Actin filaments were demonstrated in the stereocilia and their rootlets, in the cuticular plate and in relation to the zonula adherens surrounding the top of the cell. Filaments which run parallel to the cell surface were found in the cuticular plate and zonula adherens. Some filaments associated with the zonula adherens had a functional orientation opposite to that of more centrally located filaments in the cuticular plate. A structural complex consisting of a solid filament surrounded by actin filaments in hexagonal packing was found in the periphery of the cuticular plate. The possibility is suggested that the central filament is myosin.
Similar content being viewed by others
References
Begg, D. A., Rodewald, R. &Rebhun, L. I. (1978) The visualization of actin filament polarity in thin sections. Evidence from the uniform polarity of membrane-associated filaments.Journal of Cell Biology 79, 846–52.
Bretscher, A. &Weber, K. (1978) Localization of actin and microfilament-associated protein in the microvilli and terminal web of the intestinal brush border by immunofluorescence microscopy.Journal of Cell Biology 79, 838–45.
Carlsson, L., Nyström, L.-E., Sundqvist, T., Markey, F. &Lindberg, V. (1977) Actin polymerizability influenced by profilin, a low molecular weight protein in non-muscle cells.Journal of Molecular Biology 115, 465–83.
Clarke, M. &Spudich, J. A. (1977) Nonmuscle contractile proteins: The role of actin and myosin in cell motility and shape determination.Annual Review of Biochemistry 46 797–822.
Derosier, D., Mandelkow, E., Silliman, A., Tilney, L. &Kane, R. (1977) Structure of actin-containing filaments from two types of nonmuscle cells.Journal of Molecular Biology 113, 679–95.
Drenckhahn, D. &Gröschel-Stewart, U. (1977) Localization of myosin and actin in ocular nonmuscle cells. Immunofluorescence-microscopic, biochemical and electronmicroscopic studies.Cell and Tissue Research 181, 493–503.
Everitt, E. &Philipson, L. (1974) Structural proteins of adenoviruses. XI. Purification of three low molecular weight virion proteins of adenovirus type 2 and their synthesis during productive infection.Virology 62, 253–69.
Flock, A. (1977) Physiological properties of sensory hairs in the ear. InPsychophysics and Physiology of Hearing (edited byEvans, E. F. andWilson, J. P.), pp. 15–26. New York: Academic Press.
Flock, A. &Cheung, H. (1977) Actin filaments in sensory hairs of the inner ear receptor cells.Journal of Cell Biology 75, 339–43.
Flock, A., Flock, B. &Murray, E. (1977) Studies on the sensory hairs of receptor cells in the inner ear.Acta oto-laryngologica 83, 85–91.
Gibbons, I. R. &Gibbons, B. H. (1974) The fine structure of axonemes from sea urchin sperm flagella.Journal of Cell Biology 63, 110a (abstract).
Goldman, R. D., Chojnacki, B. &Yerna, M. J. (1979) Ultrastructure of microfilament bundles in baby hamster kidney (BHK-21) cells. The use of tannic acid.Journal of Cell Biology 80, 759–66.
Griffith, L. M. &Pollard, T. D. (1978) Evidence for actin filament-microtubule interaction mediated by microtubule associated proteins.Journal of Cell Biology 78, 958–65.
Hitchcock, S. E. (1977) Regulation of motility in non-muscle cells.Journal of Cell Biology 74, 1–15.
Hudspeth, A. J. &Jacobs, R. (1979) Stereocilia mediate transduction in vertebrate hair cells.Proceedings of the National Academy of Science (U.S.A.) 76, 1506–9.
Hull, B. &Staehelin, L. A. (1979) The terminal web. A revaluation of its structure and function.Journal of Cell Biology 81, 67–82.
Ishikawa, H., Bischoff, R. &Holtzer, H. (1969) Formation of arrowhead complexes with heavy mero-myosin in a variety of cell types.Journal of Cell Biology 43, 312–28.
Johnson, G. D., Holborow, E. J. &Glynn, L. E. (1965) Antibody to smooth muscle in patients with liver disease.Lancet II, 878–90.
Korn, E. D. (1978) Biochemistry of actomyosin-dependent cell motility.Proceedings of the National Academy of Science (U.S.A.) 75, 588–99.
Lidman, K., Biberfeld, G., Fagraeus, A., Norberg, R., Thorstenson, R., Utter, R., Carlsson, L., Luca, J. &Lindberg, U. (1976) Anti-actin specificity of human smooth muscle antibodies in chronic active hepatitis.Clinical and Experimental Immunology 24, 266–72.
Lindberg, U., Carlsson, L., Markey, F. &Nyström, L. E. (1979) The unpolymerized form of actin in nonmuscle cells. InMethods and Achievements in Experimental Pathology (edited byJasmin, G. andCantin, M.), pp. 143–170. Basel: Karger.
Maizel, J. V. (1971) Polyacrylamid gel electrophoresis of viral protein.In Methods in Virology, Vol. 5 (edited byMaramocosch, K. andKoprowski, M.), pp. 170–246. New York: Academic Press.
Margossian, S. S. &Lowey, S. (1973) Structure of the myosin molecule. IV. Interaction of myosin and its subfragment with adenosine triphosphate and F-actin.Journal of Molecular Biology 74, 313–30.
Maupin-Szamier, P. &Pollard, T. D. (1978) Actin filament destruction by osmium tetroxide.Journal of Cell Biology 77, 837–52.
Metuzals, J. &Tasaki, I. (1978) Subaxolemmal filamentous network in the giant nerve fiber of the squid (Loligo pealei L.) and its possible role in excitability.Journal of Cell Biology 78, 597–621.
Mitsuhira, V. &Futaesaky, Y. (1971) On the new approach of tannic acid and digitonine to the biological fixatives.Proceedings of the Electron Microscopical Society of America 29, 494–5.
Mooseker, M. S. (1976) Brush border motility. Microvillar contraction in triton-treated brush borders isolated from intestinal epithelium.Journal of Cell Biology 71, 417–33.
Mooseker, M. S., Pollard, T. D. &Fujiwara, K. (1978) Characterization and localization of myosin in the brush border of intestinal epithelial cells.Journal of Cell Biology 79, 444–53.
Mooseker, M. S. &Tilney, L. G. (1975) Organization of an actin filament-membrane complex.Journal of Cell Biology 67, 725–43.
Perry, S. V. (1976) Closing remarks. InContractile Systems in Non-muscle Tissues (edited byPerry, S. V., Margareth, A. andAdelstein, R. S.), pp. 353–358. Amsterdam: North-Holland.
Reynolds, E. S. (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy.Journal of Cell Biology 17, 208–13.
Rodewald, R., Newman, S. B. &Karnovsky, M. J. (1976) Contraction of isolated brush borders from the intestinal epithelium.Journal of Cell Biology 70, 541–4.
Simionescu, N. &Simionescu, M. (1976) Galloylglucoses of low molecular weight as a mordant in electron microscopy. I. Procedure and evidence for mordanting effect.Journal of Cell Biology 70, 608–21.
Small, J. V. (1977) Studies on isolated smooth muscle cells: the contractile apparatus.Journal of Cell Science 24, 327–49.
Thuneberg, L. &Røstgaard, J. (1969) Motility of microvilli. A film demonstration.Journal of Ultrastructure Research 29, 578a.
Tilney, L. G. (1975) Actin filaments in the acrosomal reaction ofLimulus sperm.Journal of Cell Biology 64, 289–310.
Watson, M. L. (1958) Staining of tissue sections for electron microscopy with heavy metals.Journal of Biophysical and Biochemical Cytology 4, 375–479.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Flock, Å., Cheung, H.C., Flock, B. et al. Three sets of actin filaments in sensory cells of the inner ear. Identification and functional orientation determined by gel electrophoresis, immunofluorescence and electron microscopy. J Neurocytol 10, 133–147 (1981). https://doi.org/10.1007/BF01181749
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01181749