Summary
A histochemical study of the ultrastructural localization of ATPases in cultures of chick embryo spinal cord has been carried out. The localization of Ca2+ and Mg2+ activated membrane ATPases appears similar: both enzyme activities are localized on the outer surfaces of plasma membranes of all kinds of cell present in the cultures, with the exception of the membranes in direct contact with the culture medium. The results are discussed in relation to data concerning the localization and function of ATPasesin vivo and in relation to the possible establishment of mechanisms of nutrient uptake and transfer in cultures of nervous tissue.
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Ciani, F., Contestabile, A., Minelli, G. andQuaglia, A. (1973) Ultrastructural localization of alkaline phosphatase in cultures of nervous tissuein vitro.Journal of Neurocytology 2, 105–16.
Contestabile, A., Minelli, G. andCiani, F. (1973) Enzymic activities in dissociated neurons differentiated in culturesin vitro.Journal of Neurochemistry 20, 691–8.
Farquhar, M. G. andPalade, G. E. (1966) Adenosine triphosphatase localization in amphibian epidermis.Journal of Cell Biology 30, 359–79.
Forbes, M. S. andSperelakis, N. (1972) (Na+, K+)-ATPase activity in tubular systems of mouse cardiac and skeletal muscles.Zeitschrift für Zellforschung und mikroskopische Anatomie 134, 1–11.
Friede, R. L. (1964) The enzymatic response of astrocytes to various ions in vitro.Journal of Cell Biology 20, 5–15.
Friede, R. L. (1966)Topographic Brain Chemistry. New York: Academic Press.
Hermetet, J. C., Treska, J. andMandel, P. (1970) Histochemical study of isolated neurons in culture from chick embryo sympathetic ganglia.Histochemie 22, 177–86.
Kim, S. U. (1966) Histochemical demonstration of oxidative enzymes associated with carbohydrate metabolism in cerebellar neurons culturedin vitro.Arcbivum Histologicum Japonicum 27, 465–71.
Kim, S. U. (1970) Cytochemical demonstration of ‘marker’ enzymes in nerve cells culturedin vitro.Experientia 26, 292–3.
Leherer, G. M., Bornstein, M. B., Weiss, C., Furman, M. andLichtman, C. (1970) Enzymes of carbohydrate metabolism in the rat cerebellum developingin situ andin vitro.Experimental Neurology 27, 410–25.
Minelli, G., Ciani, F. andContestabile, A. (1971) The occurrence of some enzymatic activities in the differentiation of nerve tissue ofGallus embryo in culturesin vitro.Histochemie 28, 160–9.
Minelli, G., Quaglia, A., Contestabile, A. andCiani, F. (1972) Acetylcholinesterase fine localization in culturesin vitro of chick embryo nervous tissue.Journal of Submicroscopical Cytology 4, 45–55.
Moses, H. L. andRosenthal, A. S. (1968) Pitfalls in the use of lead ions for histochemical localization of nucleoside phosphatases.Journal of Histochemistry and Cytochemistry 16, 530–39.
Murray, M. R. (1965) Nervous tissuein vitro. InCells and Tissue in Culture (edited byWillmer, E. N.), pp. 373–455. New York: Academic Press.
Pearse, A. G. E. (1968)Histochemistry. Theoretical and Applied. Vol. I, London: Churchill Livingstone.
Rostgaard, J. andBehnke, O. (1965) Fine structural localization of adenosine nucleoside phosphatase activity in the sarcoplasmic reticulum and the T system of rat myocardium.Journal of Ultrastructure Research 12, 579–91.
Reynolds, E. S. (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy.Journal of Cell Biology 17, 208–12.
Samorajski, T. andMcCloud, J. (1961) Alkaline phosphomonoesterase and blood-brain permeability.Laboratory Investigation 10, 492–501.
Schulze, W. andWollenberger, A. (1969) Zytochemische Lokalisation und Differenzierung von Na+-K+ und anderer Membranstandiger ATPase-Aktivitat in Herzmuskel.Histochemie 19, 302–18.
Sensenbrenner, M., Springer, N., Booher, J. andMandel, P. (1972) Histochemical studies during differentiation of dissociated nerve cells cultivated in the presence of brain extracts.Neurobiology 2, 49–60.
Sensenbrenner, M., Booher, J., Mandel, P. andKnoegten, M. F. (1973) Histochemical study of dissociated nerve cells from embryonic chick cerebral hemispheres in flask cultures.Experientia 29, 699–701.
Shapiro, D. L. andSchrier, B. K. (1973) Cell cultures of fetal rat brain. Growth and marker enzyme development.Experimental Cell Research 77, 239–47.
Torack, R. M. andBarrnett, R. J. (1963) Nucleoside phosphatase activity in membranous fine structures of neurons and glia.Journal of Histochemistry and Cytochemistry 11, 763–72.
Torack, R. M. andBarrnett, R. J. (1964) The fine structural localization of nucleoside phosphatase in structures comprising the blood brain barrier.Journal of Neuropathology and Experimental Neurology 23, 46–9.
Tunnicliff, G. andKim, S. U. (1973) Synaptogenesis and the development of neurotransmitter enzymes in organotypic cultures of chick spinal cord.Brain Research 49, 410–6.
Wachstein, M. andMeisel, E. (1957) Histochemistry of hepatic phosphatases at a physiological pH with special reference to the demonstration of bile canaliculi.American Journal of Clinical Pathology 27, 13–23.
Werner, I., Peterson, G. R. andShuster, R. (1971) Choline acetyltransferase and acetylcholinesterase in cultured brain cells from chick embryos.Journal of Neurochemistry 18, 141–51.
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Quaglia, A., Minelli, G., Ciani, F. et al. The fine localization of ATPases in culturesin vitro of chick embryo spinal cord. J Neurocytol 5, 661–667 (1976). https://doi.org/10.1007/BF01181579
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DOI: https://doi.org/10.1007/BF01181579