Abstract
The intracellular localisation of phytochrome in oat (Avena sativa L. cv. Garry Oat) coleoptiles was analysed by electron microscopy. Serial ultrathin sections of resin-embedded material were indirectly immunolabeled with polyclonal antibodies against phytochrome together with a gold-coupled second antibody. The limits of detectability of sequestered areas of phytochrome (SAPs) were analysed as a function of light pretreatments and amounts of the far-red absorbing form of phytochrome (Pfr) established. In 5-d-old dark-grownAvena coleoptiles SAPs were not detectable if less than 13 units of Pfr — compared with 100 units total phytochrome of 5-d-old dark-grown seedlings — were established by a red light pulse. In other sets of experiments, seedlings were preirradiated either with a non-saturating red light pulse to allow destruction to occur or with a saturating red followed by a far-red light pulse to induce first SAP formation and then its disaggregation. These preirradiations resulted in an increase of the limit of detectability of SAP formation after a second red light pulse to 38–41 and 19–23 units Pfr, respectively. We conclude that with respect to Pfr-induced SAP formation an adaptation process exists and that our data indicate that SAP formation is not a simple self-aggregation of newly formed Pfr.
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Abbreviations
- FR:
-
far-red light
- Pfr, Pr:
-
far-red-absorbing and red-absorbing forms of phytochrome, respectively
- Plot:
-
total phytochrome (Pfr + Pr)
- R:
-
red light
- SAP:
-
sequestered areas of phytochrome
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This work was supported by Deutsche Forschungsgemeinschaft (SFB 206). The competent technical assistance of Karin Fischer is gratefully acknowledged.
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Hofmann, E., Speth, V. & Schäfer, E. Intracellular localisation of phytochrome in oat coleoptiles by electron microscopy. Planta 180, 372–377 (1990). https://doi.org/10.1007/BF01160392
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DOI: https://doi.org/10.1007/BF01160392