Skip to main content
SpringerLink
Log in

Gamma-glutamyltransferase induction by dexamethasone in cytochrome P-450-depleted rat liver

  • Published:
Bioscience Reports

Abstract

The effects of the cytochrome P-450 depletion by cobaltic protoporphyrin IX on the postnatal glucocorticoid-inducibility of the membrane-bound enzyme gamma-glutamyltransferase have been assessed in the rat liver. Dexamethasone-induced gamma-glutamyltransferase activity in 14-, 28- and 77-day-old rats was high, weak and absent, respectively, and inversely correlated with the physiological cytochrome P-450 activity. In the liver acinus, the enzyme was reexpressed by the zone 1 and zone 2 hepatocytes in suckling rats, substantially only by the zone 1-hepatocytes in just weaned rats. Following cytochrome P-450 depletion, gamma-glutamyltransferase induction by dexamethasone was more rapid, more intense and more extended in the liver, acinus, occurring also in the zone 3 hepatocytes in suckling rats, in the zone 2 and a few zone 3 hepatocytes in just weaned rats. Further, the enzyme induction occurred also in adult rats in the zone 1 and in some zone 2 cells. This shows that cytochrome P-450 modulates the extent of hepatic gamma-glutamyltransferase induction by dexamethasone in postnatal rat-hepatocytes. The phenomenon may be consequent on hormone biotransformation changes caused by the cytochrome P-450 depletion.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Meister, A. (1981) In:Current Topics in Cellular Regulation (Eastbrook, R. W. and Srere, P., eds.). Academic Press, New York, London, Toronto, Sydney, San Francisco, pp. 21–58.

    Google Scholar 

  2. Tate, S. S. (1980) In:Enzymatic Basis of Detoxification (Jacoby, W. B., ed.). Vol. 2. Academic Press, New York, pp. 95–120.

    Google Scholar 

  3. Meredith, M. J. and Williams, G. M. (1986)J. Biol. Chem. 261:4986–4992.

    Google Scholar 

  4. Wapnir, R. A., Mancusi, J. and Goldstein, L. A. (1982)Experientia (Basel) 38:647–648.

    Google Scholar 

  5. Iannaccone, P. M. and Koizumi, J. (1983)J. Histochem. Cytochem. 31:1312–1316.

    Google Scholar 

  6. Tongiani, R., Paolicchi, A. and Chieli, E. (1984)Biosci. Rep. 4:203–211.

    Google Scholar 

  7. Tongiani, R. and Paolicchi, A. (1989)Acta histochem. 86:51–61.

    Google Scholar 

  8. Kitagawa, T., Imai, F., and Sato, K. (1980)Jap. J. Cancer Res. (Gann) 71:362–366.

    Google Scholar 

  9. Bone, III, S. N., Michalopoulos, G. and Jirtle, R. L. (1985)Cancer Res. 45: 1222–1228.

    Google Scholar 

  10. Ronchi, G. and Dosmet, V. J. (1973)Beitr. Pathol. 150:316–321.

    Google Scholar 

  11. Colombo, J. P. and Gigon, P. L. (1979)Experientia (Basel) 35:1005–1006.

    Google Scholar 

  12. Farber, E. and Cameron, R. (1980)Adv. Cancer Res. 31:125–226.

    Google Scholar 

  13. Farber, E. (1984)Can. J. Biochem. Cell. Biol. 62:486–494.

    Google Scholar 

  14. Roomi, M. W., Ho, R. K., Sarma, D. S. R. and Farber, E. (1985)Cancer Res. 45: 564–571.

    Google Scholar 

  15. Lebsanft, J., Luippold, G. and Schwarz, L. R. (1985)Cancer Lett. 29:29–36.

    Google Scholar 

  16. Cheeseman, K. H., Albano, E. F., Tomasi, A. and Slater, T. F. (1984)Chem.-Biol. Interactions 50:143–151.

    Google Scholar 

  17. Omura, T. and Sato, R. (1964)J. Biol. Chem. 239:2370–2378.

    Google Scholar 

  18. Prough, R. A., Burke, M. D. and Mayer, R. T. (1979) In:Methods in Enzymology (Fleischer, S. and Packer, L., eds.). Vol. 52. Academic Press: New York, San Francisco, London, pp. 372–377.

    Google Scholar 

  19. Huseby, N. E. and Strömme, J. H. (1979)Scandinav. J. Clin. Lab. Invest. 34:357–363.

    Google Scholar 

  20. Ogawa, K., Solt, D. B. and Farber, E. (1980)Cancer Res. 40:725–733.

    Google Scholar 

  21. Rutenburg, A. M., Kim, H., Fischbein, J. W., Hanker, J. S., Wasserkrug, H. L. and Seligman, A. M. (1969)J. Histochem. Cytochem. 17:517–526.

    Google Scholar 

  22. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951)J. Biol. Chem. 193:265–275.

    Google Scholar 

  23. Rappaport, A. M. (1976)Beitr. Pathol. 157:215–243.

    Google Scholar 

  24. Kato, R., Vassanelli, P., Frontino, G. and Chiesara, E. (1964)Biochem. Pharmacol. 13:1037–1051.

    Google Scholar 

  25. Baron, J., Redick, J. A. and Guengerich, F. P. (1978)Life Sci. 23:2627–2632.

    Google Scholar 

  26. Fiala, S. and Fiala, E. S. (1973)J. Natl. Cancer Inst. 51:151–158.

    Google Scholar 

  27. Sirica, A. E., Richards, W., Tsukada, Y., Sattler, C. A. and Pitot, H. C. (1979)Proc. Natl. Acad. Sci. USA,76:283–287.

    Google Scholar 

  28. Tongiani, R., Paolicchi, A. and Chieli, E. (1987)Acta histochem 82:137–148.

    Google Scholar 

  29. Tongiani, R., Paolicchi, A. and Nöhammer, G. (1987)Bas. Appl. Histochem. 31:441–454.

    Google Scholar 

  30. Smith, M. T., Loveridge, N., Wills, E. D. and Chayen, J. (1979)Biochem. J. 182:103–108.

    Google Scholar 

  31. Neal, G. E., Metcalf, S. A., Legg, R. F., Judah, D. J. and Green, J. A. (1981)Carcinogenesis 2:457–461.

    Google Scholar 

  32. El Mouelhi, M., Didolkar, M. S., Elias, E. G., Guengerich, F. P. and Kauffman, F. C. (1987)Cancer Res. 47:460–466.

    Google Scholar 

  33. Meister, A. M. and Andersen, M. E. (1983)Annu. Rev. Biochem. 52:711–760.

    Google Scholar 

  34. Okomoto, K., Isohashi, F., Tsukanaka, K., Oriuchi, M. and Sakamoto, Y. (1983)Endocrinology 112:336–340.

    Google Scholar 

  35. Voigt, J. and Sekeris, C. E. (1980)Biochim. Biophys. Acta 633:422–435.

    Google Scholar 

  36. Omrani, G. R., Furukawa, H., Sherwood, J. A. and Loeb, J. N. (1983)Endocrinology 112:178–186.

    Google Scholar 

  37. Waxman, D. J. (1988)Biochem. Pharmacol. 37:71–84.

    Google Scholar 

  38. Gonzales, F. J. (1989)Pharmacol. Rev. 40:243–288.

    Google Scholar 

  39. Simmons, D. L., McQuiddy, P. and Kasper, C. B. (1987)J. Biol. Chem. 262:326–332.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Istituto di Patologia Generale, Università di Pisa, Scuola Medica, via Roma 55, I-56126, Pisa, Italy

    Roberto Tongiani, Aldo Paolicchi & Elisabetta Chieli

Authors
  1. Roberto Tongiani
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Aldo Paolicchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Elisabetta Chieli
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tongiani, R., Paolicchi, A. & Chieli, E. Gamma-glutamyltransferase induction by dexamethasone in cytochrome P-450-depleted rat liver. Biosci Rep 10, 437–444 (1990). https://doi.org/10.1007/BF01152290

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01152290

Key Words

Search

Navigation