Summary
Inductive action of duodenal mesenchyme on stomach endoderm in the chick embryo was chronologically analysed in vitro by the use of electron microscopy and immunofluorescence techniques. The behaviour of the endoderm-mesenchyme interfaces was particularly studied during the induction. In recombinates of 4-day stomach endoderm and 6-day duodenal mesenchyme, all the endodermal cells were undifferentiated at the start of cultivation. Small-intestinal sucrase antigen could first be detected on the 5th day of cultivation in one-third of the stomach endoderm, and a striated border on the 7th day. With a longer cultivation period, intestine-type cells increased in number in the stomach endoderm and the density of microvilli on the apical surface became higher. At the endoderm-mesenchyme interfaces a number of direct contacts between endodermal and mesenchymal cells were observed from the beginning to the end of cultivation. These were especially abundant in the early period before the appearance of signs of intestinal cytodifferentiation. These results suggest that the mesenchymal cells adjacent to the endodermal tissue play an important role in the intestinal induction which occurs during the early period of cultivation, probably via direct cell-to-cell contracts.
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References
Bluemink JG, Van Maurik P, Lawson KA (1976) Intimate cell contacts at the epithelial-mesenchymal interface in embryonic mouse lung. J Ultrastruct Res 55:257–270
Csato W, Merker HJ (1983) Production and formation of the basement membrane in embryonic tissues of the mouse. Cell Tiss Res 228:85–98
Cunha GR, Fujii H, Neubauer BL, Shannon JM, Sawyer L, Reese BA (1983) Epithelial-mesenchymal interactions in prostatic development. I. Morphological observations of prostatic induction by urogenital sinus mesenchyme in epithelium of the adult rodent urinary bladder. J Cell Biol 96:1662–1670
Cutler LS, Chaudhry AP (1973) Intercellular contacts at the epithelial-mesenchymal interface during the prenatal development of rat submandibular gland. Dev Biol 33:229–240
Dhouailly D, Rogers GE, Sengel D (1978) The specification of feather and scale protain synthesis in epidermal-dermal recombinations. Dev Biol 65:58–68
Haffen K, Kedinger M, Simon-Assmann PM, Lacroix B (1982) Mesenchyme-dependent differentiation of intestinal brushborder enzymes in the gizzard endoderm of the chick embryo. In: Burger MM, Weber R (eds) Embryonic development. Part B: Cellular aspects. Liss AL, New York, pp 261–270
Ishizuya A (1980) Electron microscopical study of the development of the quail intestinal epithelium J Fac Sci Univ Tokyo Sect IV 14:335–344
Ishizuya A (1981) Epithelial differentiation of the intestine of young quail embryos cultured organotypically in vitro. J Fac Sci Univ Tokyo Sect IV 15:71–79
Ishizuya-Oka A (1983) Electron microscopical study of self-differentiation potency in the chick embryonic endoderm cultured in vitro. Wilhelm. Roux's Arch 192:171–178
Ishizuya-Oka A, Mizuno T (1984) Intestinal cytodifferentiation in vitro of chick stomach endoderm induced by the duodenal mesenchyme. J Embryol Exp Morphol 82:163–176
Koller EJ, Fisher C (1980) Tooth induction in chick epithelium: expression of quiescent genes for enamel synthesis. Science 207:993–995
Masui T (1982) Intestinalization of the area-vitellina endoderm cultured in association with digestive-tract mesenchymes J Embryol Exp Morphol 72:117–124
Mathan M, Hermos JA, Trier JS (1972) Structural features of the epithelio-mesenchymal interface of rat duodenal mucosa during development. J Cell Biol 52:577–588
Matsushita S (1983) Purification and partial characterization of chick intestinal sucrase. Comp Biochem Physiol B 76:465–470
Merker HJ, Barrach HJ (1981) The morphology of basement membrane formation. Eur J Cell Biol 26:111–120
Mizuno T, Ishizuya A (1982) Etude ultrastructurale de l'embryon de poulet après autodifférenciation in vitro. CR Séances Soc Biol 176:889–893
Overton J, Shoup J (1964) Fine structure of cell surface specialization in maturing duodenal mucosa of the chick. J Cell Biol 21:75–95
Sawyer RH, Fallon JF (1983) Epithelial-mesenchymal interactions in development. Praeger, New York
Sawyer RH, O'Guin WH, Knapp LW (1984) Avian scale development. X. Dermal induction of tissue-specific keratins in extraembryonic ectoderm. Dev Biol 101:8–18
Saxén L, Lehtonen E (1978) Transfilter induction of kidney tubules as a function of the extent and duration of intercellular contacts. J Embryol Exp Morphol 47:97–109
Saxén L, Ekblom P, Thesleff I (1980) Mechanisms of morphogenetic cell interactions. In: Johnson MH (ed) Development in mammals, vol. 4. Elsevier, Amsterdam, pp 161–202
Sigot M (1971) Organogenèse de l'estomac de l'embryon de Poulet. Analyse des mécanismes de la différenciation. Arch Anat Microsc Morphol Exp 60:169–204
Slavkin HC, Bingas PJ (1976) Epithelial-mesenchyme interaction during odontogenesis. IV. Morphological evidence for direct heterotypic cell-cell-contacts. Dev Biol 50:428–442
Thesleff I, Hurmerinta K (1981) Tissue interactions in tooth development. Differentiation 18:75–88
Wessells NK (1977) Tissue interactions and development. W. A. Benjamin, Menlo Park, California
Wolff E, Haffen K (1952) Sur une méthode, de culture d'organes embryonnaires “in vitro”. Tex Rep Biol Med 10:463–472
Yasugi S, Mizuno T (1984) Différenciation hétérotypique de l'hypoblaste d'oiseau sous l'influence du mésenchyme proventriculaire. CR Séances Soc Biol 178:580–583
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Ishizuya-Oka, A., Mizuno, T. Chronological analysis of the intestinalization of chick stomach endoderm induced in vitro by duodenal mesenchyme. Wilhelm Roux' Archiv 194, 301–305 (1985). https://doi.org/10.1007/BF01152176
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DOI: https://doi.org/10.1007/BF01152176