Summary
A quantitative light microscopic study has been carried out on the myenteric and submucosal ganglia of the stomach, duodenum, ileum, proximal colon and rectum of the guinea-pig; the enteric ganglia of the ileum were studied also in the mouse, rabbit and sheep. The area of the profiles of nerve cells, of nerve cell nuclei and of glial nuclei, and the proportion of the area of ganglia occupied by neuropil were measured, and the relative numbers of neurons and glial cells were estimated. The myenteric ganglia were found to be firmly anchored to the stroma of the muscle coat; their shape and the shape of their component cells varied with contraction and distension of the musculature. The range of neuronal sizes in the myenteric ganglia was extremely wide. In the guinea-pig, the myenteric neurons were on average largest in the stomach and duodenum and smallest in the ileum, with intermediate values in the colon and rectum; the submucosal neurons showed little variation in average size along the length of the gut. The average size of ganglion neurons in the ileum was greatest in the sheep and smallest in the mouse, and had intermediate values in the guinea-pig and rabbit. The percentage volume of neuropil in the myenteric ganglia was 51% in the mouse, 65% in the guinea-pig, 70% in the rabbit, and 74% in the sheep. The number of glial cells relative to the number of neurons was also ranked in the same order. In all the species examined the submucosal ganglia, when compared with the corresponding myenteric ganglia, had a smaller percentage volume of neuropil, a much smaller number of glial cells and (except in the mouse ileum) neurons of smaller average size. In all the ganglia there was a positive correlation between size of neurons and size of glial cells. The results are discussed in the light of possible relations between body size (and length of the intestine), numerical density of ganglion neurons, average size of neurons, amount of musculature, average distance between neurons, and amount of neuropil.
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References
Dupont, J. -R., Jervis, H. R. &Sprinz, H. (1965) Auerbach's plexus of the rat cecum in relation to the germfree state.Journal of Comparative Neurology 125, 11–18.
Furness, J. B. &Costa, M. (1980) Types of nerves in the enteric nervous system.Neuroscience 5, 1–20.
Gabella, G. (1971) Neuron size and number in the myenteric plexus of the newborn and adult rat.Journal of Anatomy 109, 81–95.
Gabella, G. (1981) On the musculature of the gastro-intestinal tract of the guinea-pig.Anatomy and Embryology 163, 135–56.
Gabella, G. (1982) Structure of muscles and nerves in the gastrointestinal tract. InPhysiology of the Gastrointestinal Tract (edited byJohnson, L. L.), pp. 197–241. New York: Raven Press.
Gabella, G. (1984) Size of neurons and glial cells in the intramural ganglia of the hypertrophic intestine of the guinea-pig.Journal of Neurocytology 13, 73–84.
Gershon, M. D. &Erde, S. M. (1981) The nervous system of the gut.Gastroenterology 80, 1571–94.
Gunn, M. (1968) Histological and histochemical observations on the myenteric and submucous plexuses of mammals.Journal of Anatomy 102, 223–39.
Hendry, I. A. &Campbell, J. (1976) Morphometric analysis of rat superior cervical ganglion after axotomy and nerve growth factor treatment.Journal of Neurocytology 5, 351–60.
Henneman, E., Somjen, G. &Carpenter, D. O. (1964) Functional significance of cell size in spinal motoneurons.Journal of Neurophysiology 28, 560–80.
Irwin, D. A. (1931) The anatomy of Auerbach's plexus.American Journal of Anatomy 49, 141–66.
Konigsmark, B. W. (1970) Methods for the counting of neurons. InContemporary Research Methods in Neuroanatomy (edited byNauta, W. J. H. andEbbeson, S. O. E.), pp. 315–80. New York: Springer Verlag.
Matsuo, H. (1934) A contribution on the anatomy of Auerbach's plexus.Japanese Journal of Medical Sciences. I. Anatomy 4, 417–28.
Matthews, M. R. (1974) Ultrastructure of ganglionic junctions. InThe Peripheral Nervous System (edited byHubbard, J. I.) pp. 111–50. New York: Plenum Press.
Ohkubo, K. (1936a) Studies on the intrinsic nervous system of the digestive tract. The submucous plexus of the guinea-pig.Japanese Journal of Medical Sciences. I. Anatomy 6, 1–20.
Ohkubo, K. (1936b) Studien über das intramurale Nervensystem des Verdauungskanals. II. Die Plexus myentericus und Plexus subserosus des Meerschweinchens.Japanese Journal of Medical Sciences. I. Anatomy 6, 21–37.
Prestige, M. C. (1974) Axon and cell numbers in the developing nervous system.British Medical Bulletin 30, 107–11.
Stöhr, P. Jr (1930) Mikroskopische Studien zur Innervation des Magen-Darmkanales.Zeitschrift für Zellforschung und mikroskopische Anatomie 12, 66–154.
Tafuri, W. I. (1957) Auerbach's plexus in the guinea-pig. I. A quantitative study of the ganglia and nerve cells in the ileum, caecum and colon.Acta anatomica 31, 522–30.
Tafuri, W. L. &Almeida Campos, F. D. (1958) Der Auerbachsche Plexus bei der Maus.Zeitschrift für Naturforschung 13b, 816–19.
Wilson, A. J., Furness, J. B. &Costa, M. (1981) The fine structure of the submucous plexus of the guinea-pig ileum. I. The ganglia, neurons, Schwann cells and neuropil.Journal of Neurocytology 10, 759–84.
Wood, J. D. (1981) Physiology of the enteric nervous system. InPhysiology of the Gastrointestinal Tract (edited byJohnson, L. L.), pp. 1–37. New York: Raven Press.
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Gabella, G., Trigg, P. Size of neurons and glial cells in the enteric ganglia of mice, guinea-pigs, rabbits and sheep. J Neurocytol 13, 49–71 (1984). https://doi.org/10.1007/BF01148318
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DOI: https://doi.org/10.1007/BF01148318