Conclusions
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1.
Sensitivity of chromatin DNA of rat liver to DNase I and the level of its methylation do not change noticeably 3.5 h after administration of an inducing dose of hydrocortisone to animals.
-
2.
Loci activated by glucocorticoids must initially possess potentially active conformation sensitive to DNase I.
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Literature cited
G. A. Romanov, N. A. Romanova, V. B. Rozen, et al., Mol. Biol.,15, No. 4, 857–874 (1981).
F. Payvar, O. Wrange, J. Carlstedt-Duke, et al., Proc. Natl. Acad. Sci. USA,78, 6628–6632 (1981).
J. G. Compton, W. T. Schrader, and B. W. O'Malley, Biochem. Biophys. Res. Commun.,105, 96–104 (1982).
E. R. Mulvihill, J.-P. LePennec, and P. Chambon, Cell,28, 621–632 (1982).
A. P. Kuz'menko, V. S. Dashkevich, G. A. Romanov, et al., Dokl. Akad. Nauk SSSR,272, No. 3, 739–741 (1983).
R. T. Simpson, in: Gene Amplification and Analysis, Vol. 2, New York (1981), pp. 347–368.
B. F. Vanyushin, Usp. Biol. Khimii,24, 15 (1983).
G. Schütz and P. Feigelson, Anal. Biochem.,46, 149–155 (1972).
G. A. Romanov, N. A. Sokolova, V. B. Rozen, et al., Biokhimiya, No. 12, 2140–2149 (1976).
A. S. Spirin, Biokhimiya, No. 5, 656–662 (1958).
K. Burton, Methods Enzymol.,12, 163 (1968).
G. Schütz, L. Killewich, G. Chen, et al., Proc. Natl. Acad. Sci. USA,75, 1017–1020 (1975).
W. G. Roewekamp. E. Hofer, and C. E. Sekeris, Eur. J. Biochem.,70, 259–268 (1976).
F. Kenney and F. Kull, Proc. Natl. Acad. Sci. USA,50, 493 (1963).
H. Grote, J. Voigt, and C. E. Sekeris, Biochem. J.,212, 305–312 (1983).
G. S. Arbuzova, I. M. Gryaznova, T. M. Morozova, et al., Mol. Biol.,2, No. 3, 308–312 (1968).
R. I. Salganik, T. M. Morozova, and N. A. Zakharov, Biochim. Biophys. Acta, 174, 755–757 (1969).
L. K. Johnson and J. D. Baxter, J. Biol. Chem.,253, 1991–1997 (1978).
V. V. Adler, M. A. Krasil'nikov, G. Yu. Bochkarev, et al., Biokhimiya, No. 6, 915–920 (1982).
N. A. Dudareva, V. S. Dashkevich, and R. I. Salganik, Biokhimiya, No. 7, 1305–1311 (1980).
J. C. Gasson, T. Ryden, and S. Bourgeois, Nature,302, 621–623 (1983).
S. J. Compere and R. D. Palmiter, Cell,25, 233–240 (1981).
J.-N. Lapeyre, A. L. Maizel, and F. F. Becker, Biochem. Biophys. Res. Commun.,95, 630–637 (1980).
R. D. Palmiter, E. R. Mulvihill, G. S. Mcknight, et al., Cold Spring Harbor Symp. Quant. Biol.,42, 639–647 (1978).
A. Garel and R. Axel, Proc. Natl. Acad. Sci. USA,74, 4867–4871 (1977).
M. T. Kuo, J. L. Mandel, and P. Chambon, Nucleic Acids Res.,7, 2106–2113 (1979).
A. F. Wilks, P. J. Cozens, I. W. Mattaj, et al., Proc. Natl. Acad. Sci. USA,79, 4252–4255 (1982).
G. J. Dimitriadis and J. R. Tata, Biochem. J.,202, 491–497 (1982).
J. L. Williams and J. R. Tata, Nucleic Acids Res.,11, 1151–1166 (1983).
V. B. Rozen and A. N. Smirnov, Receptors and Steroid Hormones [in Russian], Moscow (1981), pp. 65–76.
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We express our gratitude to Prof. V. B. Rozen for his interest in the work and useful comments and to V. V. Ashapkin for his assistance in conducting the individual experiments.
Translated from Problemy Éndokrinologii, Vol. 30, No. 6, pp. 38–42, November–December, 1984.
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Romanov, G.A., Zhavoronkova, E.N., Savel'ev, S.V. et al. Nuclease sensitivity and methylation of liver chromatin DNA in rats in initial period of effect of glucocorticoids. Neurosci Behav Physiol 16, 285–289 (1986). https://doi.org/10.1007/BF01148168
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DOI: https://doi.org/10.1007/BF01148168