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Somatic hybrids obtained by fusion betweenPoncirus trifoliata (2x) andFortunella hindsii (4x) protoplasts

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Abstract

Somatic hybrids were obtained by the symmetric fusion of embryogenic callus cells from tetraploid ‘Mame’ kumquat [Fortunella hindsii (Champ.) Swing.] and mesophyll cells from diploid trifoliate orange [Poncirus trifoliata (L.) Raf.]. Southern blot analysis of three regenerants revealed that they carried specific rDNA fragments from both fusion partners, thereby confirming their hybridity. In contrast, mitochondrial DNA (mtDNA) and chloroblast DNA (cpDNA) were unidirectionally transmitted from the callus parent without any evidence of recombination. No differences in the restriction fragment patterns of rDNA, mtDNA or cpDNA could be detected among the regenerants. Flow cytometry showed that two regenerants were hexaploids, as expected, but that one was pentaploid, probably due to elimination of chromosomes prior to the regeneration of this plant.

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Abbreviations

MS :

Murashige and Skoog (1962)

DIG :

digoxigenin

AMPPD :

3-(2′-spiroadamantane)-4-methoxy-4-(3″-phosphoryloxy)-phenyl-1,2-dioxetane

FACS :

fluorescence-activated cell sorter

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Authors and Affiliations

  1. Faculty of Agriculture, Tokyo University of Agriculture, 156, Setagaya-ku, Tokyo, Japan

    M. Miranda & F. Ikeda

  2. Shoda Shoyu Co., 374, Tatebayashi, Gunma, Japan

    T. Motomura

  3. Research and Developmental Division, Kikkoman Corporation, 278, Noda, Chiba, Japan

    T. Ohgawara & W. Saito

  4. Department of Citriculture, National Institute of Fruit Tree Science, Okitsu, 424-02, Shimizu, Shizuoka, Japan

    T. Endo, M. Omura & T. Moriguchi

Authors
  1. M. Miranda
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  2. T. Motomura
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  3. F. Ikeda
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  4. T. Ohgawara
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  5. W. Saito
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  6. T. Endo
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  7. M. Omura
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  8. T. Moriguchi
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Communicated by G. Phillips

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Miranda, M., Motomura, T., Ikeda, F. et al. Somatic hybrids obtained by fusion betweenPoncirus trifoliata (2x) andFortunella hindsii (4x) protoplasts. Plant Cell Reports 16, 401–405 (1997). https://doi.org/10.1007/BF01146782

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  • DOI: https://doi.org/10.1007/BF01146782

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