Abstract
Germplasm conservation of the tropical forest trees,Cedrela odorata L.,Guazuma crinita Mart., andJacaranda mimosaefolia D. Don., at above-freezing temperatures following alginate-bead encapsulation was attempted. Shoot tips excised from in vitro plantlets were encapsulated in calcium-alginate beads and stored on different substrates at 12, 20, and 25 °C. Percent viability when encapsulated shoot tips were stored on substrate containing only water solidified with 1% (wt/vol) agar was 80% after 12 months at 12°C forC. odorata, 90% after 12 months at 25°C forG. crinita, and 70% after 6 months at 20°C forJ. mimosaefolia.
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Abbreviations
- BAP :
-
6-Benzylaminopurine
- KIN :
-
6-Furfurylaminopurine
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Maruyama, E., Kinoshita, I., Ishii, K. et al. Germplasm conservation of the tropical forest trees,Cedrela odorata L.,Guazuma crinita Mart., andJacaranda mimosaefolia D. Don., by shoot tip encapsulation in calcium-alginate and storage at 12–25°C. Plant Cell Reports 16, 393–396 (1997). https://doi.org/10.1007/BF01146780
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DOI: https://doi.org/10.1007/BF01146780