Abstract
Direct somatic embryogenesis ofBegonia gracilis was achieved from microcultured laminar segments and petioles on Murashige and Skoog medium with 0.5 mg 1−1 kinetin and 2% coconut water. Somatic embryos were obtained with greater frequency from petiole explants than from leaf blade sections. Under red light (45 μmol m−2 s−1), approximately 80% of the petiole explants successfully produced somatic embryos but only 30% of the leaf blade sections responded. However, somatic embryos were significantly more abundant on responding lamina explants (60–70 embryos/leaf section) than on petioles (40–50 embryos/petiole). These trends were similar for explants kept in the dark, but overall production was lower. Somatic embryos were produced more quickly (5 weeks) from petioles than from lamina explants (8 weeks). The somatic embryos germinated to produce plantlets and subsequently shoot cultures with the same appearance as the parental clone.
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Abbreviations
- BA :
-
benzyladenine
- MS :
-
Murashige and Skoog (1962)
- NAA :
-
naphthalene acetic acid
- SE :
-
somatic embryo
References
Ammirato PV (1983) Embryogenesis. In: Evans D, Sharp WR, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture. Macmillan, New York, pp 82–123
Doskotch RW, Hufford CD (1970) Hexanor-cucurbitacin D, a degraded cucurbitacin fromBegonia tuberhybrida. Can J Chem 48:1787–1788
Doskotch RW, Makik MY, Beal JL (1968) The isolation and characterization of the antitumor principle fromBegonia tuberhybrida. Lloydia 31:424
Hernandez MR, Gally-Jorda M (1981) Plantas medicinales. Arbol, Mexico City
Huxley A (1992) Dictionary of gardening. Macmillan, New York
Hvoslef-Fide AK, Saebo A (1991) Effect of light quality on establishment of cell suspension cultures (abstract no 28). In Vitro Cell Dev Biol 27:43A
Laferriere JE (1992) Begonia as food and medicine. Econ But 46:114–116
Laferriere JE, Weber CW, Kohlhepp EA (1991) Use and nutritional composition of some traditional mountain pima plant foods. J Ethnobiol 11:93–114
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco cultures. Physiol Plant 15:473–497
Peck DE, Cumming BG (1984) In vitro propagation ofBegonia×tuberhybrida from leaf sections. HortScience 19:395–397
Rogers RB, Smith MAL (1992) Consequences of in vitro and ex vitro root initiation for miniature rose production. J Hort Sci 67:535–540
Sehgal CB (1975) In vitro differentiation of foliar embryo and adventitious buds from the leaves ofBegonia semperflorens. Ind J Exp Biol 13:486–488
Simmonds JA, Werry T (1987) Liquid-shake culture for improved micropropagation ofBegonia×hiemalis. Hort Science 22:122–124
Stefaniak B (1994) Somatic embryogenesis and plant regeneration of gladiolus (Gladiolus hort.). Plant Cell Rep 13:386–389
Takayama S (1990) Begonia. In: Ammirato PV, Evans DA, Sharp WR, Bajaj YPS (eds) Handbook of plant tissue culture. Macmillan, New York, pp 253–283
White PR (1943) A handbook of plant tissue culture. Cattell, Lancaster Penn.
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Communicated by J. M. Widholm
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Castillo, B., Smith, M.A.L. Direct somatic embryogenesis fromBegonia gracilis explants. Plant Cell Reports 16, 385–388 (1997). https://doi.org/10.1007/BF01146778
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DOI: https://doi.org/10.1007/BF01146778