Abstract
Both somatic and excised zygotic embryos of interior spruce (Picea glauca engelmannii complex) required exogenous sucrose in the medium for germination in vitro. Over a period of 29 days on sucrose-containing medium germinants with roots and epicotyls developed from both kinds of embryo, and their content of linolenic acid (9,12,15-18:3) increased about six- to eightfold. Without added sucrose, embryos showed retarded growth or were necrotic, and the content of linolenic acid was barely detectable in their fatty acid profiles. Through14C-sucrose uptake studies, it was determined that germinants consumed only 25% of the sucrose available in a 1% (wt/vol) sucrose-containing medium. Since no radiolabelled fatty acids were detected, it appears that externally supplied sucrose was not used in the synthesis of lipids. Although sucrose was present during plantlet development, 72% of the initial lipids were consumed. To some extent, the plantlets appeared to be obligate storage lipid utilizers.
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Abbreviations
- 2,4-D :
-
2,4-Dichlorophenoxyacetic acid
- FAMEs :
-
Fatty acid methyl esters
- HPLC :
-
High-performance liquid chromatography
References
Arnold S von, Erickson T (1981) In vitro studies of adventitious shoot formation inPinus contorta. Can J Bot 59:870–874
Attree SM, Pomeroy MK, Fowke LC (1992) Manipulation of conditions for the culture of somatic embryos of white spruce for improved triacylglycerol biosynthesis and desiccation tolerance. Planta 187:395–404
Attree SM, Pomeroy MK, Fowke LC (1995) Development of white spruce [Picea glauca (Moench.) Voss] somatic embryos during culture with abscisic acid and osmoticum, and their tolerance to drying and frozen storage. J Exp Bot 46:433–439
Carrier DJ, Cunningham JE, Hogge LR, Taylor DC, Dunstan DI (1995) Gas chromatography — mass spectrometry characterization of some fatty acids from white and interior spruce. J Chromatogr A 715:317–324
Ching TM (1970) Glyoxysomes in megagametophyte of germinating ponderosa pine seeds. Plant Physiol 46:475–482
Cyr DR, Webster FB, Roberts DR (1991) Biochemical events during germination and early growth of somatic embryos and seed of interior spruce (Picea glauca engelmannii complex). Seed Sci Res 1:91–97
Dunstan DI, Bekkaoui F, Pilon M, Fowke LC, Abrams SR (1988) Effects of abscisic acid and analogues on the maturation of white spruce (Picea glauca) somatic embryos. Plant Sci 58:77–84
Dunstan DI, Bethune TB, Abrams SR (1991) Racemic absciscic acid and abscisyl alcohol promote maturation of white spruce (Picea glauca) somatic embryos. Plant Sci 76:219–228
Dunstan DI, Bethune TB, Bock CA (1993) Somatic embryo maturation from long-term suspension cultures of white spruce (Picea glauca). In Vitro Cell Dev Biol 29P:109–112
Ekman R (1980) New polyenoic fatty acids in Norway spruce wood. Phytochemistry 19:147–148
Holbrook L, Magus J, Taylor DC (1992) Abscisic acid induction of elongase activity, biosynthesis and accumulation of very long chain mono-unsaturated fatty acids and oil body proteins in microspore-derived embryos ofBrassica napus L. cv Reston. Plant Sci 84:99–115
Jamieson GR, Reid EH (1972) The leaf lipids of some conifer species. Phytochemistry 11:269–275
Litvay JD, Johnson MA,Verma DC, Einspahr D, Weyrauch K (1981) Conifer suspension culture medium development using analytical data from developing seeds. Institute of Paper Chemistry Technical Paper Series no 115, Appelton, WI
Lulsdorf MM, Tautorus TE, Kikcio SI, Bethune TD, Dunstan DI (1993) Germination of encapsulated embryos of interior spruce (Picea glauca engelmannii complex) and black spruce (Picea mariana Mill.). Plant Cell Rep 12:385–389
Mohammed GH, Dunstan DI, Thorpe TA (1986) Influence of nutrient medium shoot initiation on vegetative explants excised from 15 to 18-year-oldPicea glauca. N Z J For Sci 16:297–305
Mullen RT, Gifford DJ (1995) Isocitrate lyase from germinating loblolly pine megagametophyte enzyme purification and immunocharacterization. Plant Physiol Biochem 33:87–95
Nilsson R, Liljenberg C (1991) The determination of double bond positions in poly-unsaturated fatty acids-GC/MS of the diethylamide derivative. Phytochem Anal 2:253–259
Olsson M, Nilsson R, Norberg P, Arnold S von, Liljenberg C (1994) Maturation in Norway spruce: alterations in lipid composition. Plant Physiol Biochem 32:225–232
Roberts DR, Webster FB, Flinn B, Lazaroff W, Cyr DR (1993) Somatic embryogenesis of spruce. In: Redenbaugh K (ed) Synseeds: applications of synthetic seeds to crop improvement. CRC, Boca Raton, pp 427–450
Taylor DC, Barton D, Rioux K, MacKenzie S, Reed D, Underhill E, Pomeroy K, Weber N (1992) Biosynthesis of acyl lipids containing very-long chain fatty acids in microspore derived and zygotic embryos ofBrassica napus L. cv Reston. Plant Physiol 99:1609–1618
Webster FB, Roberts DR, McInnis S, Sutton BC (1990) Propagation of interior spruce by somatic embryogenesis. Can J For Res 20:1759–1765
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Carrier, D.J., Cunningham, J.E., Taylor, D.C. et al. Sucrose requirements and lipid utilization during germination of interior spruce (Picea glauca engelmannii complex) somatic embryos. Plant Cell Reports 16, 550–554 (1997). https://doi.org/10.1007/BF01142322
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DOI: https://doi.org/10.1007/BF01142322