Abstract
Virus-transformed and tumor ceils often show a higher chemotactic activity in comparison to normal cells. Here we show that the blind-well chamber assay is an easy and rapid procedure to select cell populations with enhanced chemotactic activity. Collagen-type-specific antibodies have been applied to analyze the efficiency of cell sorting by the chemotaxis assay.
Similar content being viewed by others
References
Albini A, Richter H & Pontz BF (1983) Localization of the chemotactic active domain in fibronectin. FEBS Lett.156, 22–226.
Alitalo K, Vaheri A, Krieg T & Timpl R (1980) Biosynthesis of two subunits of type IV procollagen and of other basement membrane proteins by a human tumor cell line. European Journal of Biochemistry109, 247–255.
Gay S, Martin GR, Müller PK, Timpl R, Kühn K (1976) Simultaneous synthesis of types I and III collagen by fibroblasts in culture. Proceedings of the National Academy of Sciences, U.S.A.73, 4037–4040.
Grinnel F, Billingham L & Burgess L (1981) Distribution of fibronectin during wound healing in vivo. Journal of Investigative Dermatology76, 181–189.
Le Douarin NM (1976) Cell migration in early vertebrate development studied in interspecific chimeras, in: Embryogenesis in Mammals, pp 71–101, Ciba Foundation Symposium40 (new series), Elsevier North-Holland, Amsterdam.
Mensing H, Albini A, Krieg T, Pontz BF & Müller PK (1984) Enhanced chemotaxis of tumor-derived and virus-transformed cells to fibronectin and fibroblast-conditioned medium. International Journal of Cancer33, 43–48.
Nowack H, Hay S, Wick G, Becker U & Timpl R (1976) Preparation and use in immunohistology of antibodies specific for type I and III collagen and procollagen. J Immunol. Meth.12, 117–124.
Postlethwaite AE, Snydermann R & Kang AH (1976) The chemotactic attraction of human fibroblasts to a lymphocyte-derived factor. Journal of Experimental Medicine144, 1188–1203.
Smets LA (1980) Cell transformation as a model for tumor induction and neoplastic growth. Biochim. Biophys. Acta605, 93–111.
Timpl R (1982) Antibodies to collagens and procollagen, in: Methods in Enzymology82, 472–498.
Timpl R, Wiedemann H, Van Delden V, Furthmayr, H & Kühn K (1981) A network model for the organization of type IV collagen molecules in basement membranes. European Journal of Biochemistry120, 203–211.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Albini, A., Müller, P.K. & Parodi, S. A method to select cell populations with enhanced chemotatic activity. Biosci Rep 4, 311–318 (1984). https://doi.org/10.1007/BF01140494
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01140494