Summary
We attempted to investigate the viability of mouse blastocysts in vitro, comparing with embryo transfer to recipient mice. Despite normal appearances of the blastocysts, the rate of trophoblastic outgrowth of blastocysts fertilized in vitro was lower than that of blastocysts fertilized in vivo. However, the rate of transferred blastocysts developing into fetuses did not differ significantly between in vivo and in vitro fertilized blastocysts. It is considered that blastocyst hatching, attachment, and trophoblastic outgrowth in vitro can serve as indices for the vitality of individual embryos.
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References
Fleming TP, Pratt HPM, Braude PR: The use of mouse preimplantation embryos for quality control of culture reagents in humanin vitro fertilization programs: A cautionary note. Fertil Steril 1987;47:858–860
Caro CM, Trounson A: The effect of protein on preimplantation mouse embryo development in vitro. J Vitro Fert Embryo Transfer 1984;1:183–187
Arny M, Nachtigall L, Quagliarello J: The effect of preimplantation culture conditions on murine embryo implantation and fetal development. Fertil Steril 1987;48:861–865
Han HD, Kiessling AA:In vitro development of transferred mouse embryos conceivedin vitro in simplex and complex media. Fertil Steril 1988;50:159–163
Spindle AI, Pedersen RA: Hatching, attachment, and outgrowth of mouse blastocystsin vitro: Fixed nitrogen requirements. J Exp Zool 1972;186:305–318
Chen LT, Hsu YC: Development of mouse embryosin vitro: Preimplantation to the limb but stage. Science 1982;218:66–68
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Chida, S., Mettler, L. Screening test for mouse blastocysts as an index of the vitality of embryos. J Assist Reprod Genet 6, 310–312 (1989). https://doi.org/10.1007/BF01139188
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DOI: https://doi.org/10.1007/BF01139188