Abstract
An anti-Z-antibody-binding region between PM2-DNA map units 0.05 and 0.18, containing approx. 25% of the bound PM2 antibody molecules (1,2) has been sequenced. Analysis of this PM2 DNA sequence from map units 0.00 to 0.175 demonstrates that alternating purine/pyrimidine tracts capable of adopting the left-handed conformation are present within this antibody-binding region. Longer (GC)n-rich tracts are clustered together and comprise seven alternating purine/pyrimidine-rich areas (48%–84%) ranging from 19 to 142 nucleotides in length. The DNA located between these alternating purine/pyrimidine-rich areas exhibit a low level (0%–19%) of this sequence arrangement. There is a very strong correlation between the alternating purine/pyrimidine-rich areas and the anti-Z-DNA-IgG-binding sites. Nucleotides 1461–1583 of the PM2-DNA genome encode the bacteriophage capsid protein IV. One of the PM2 left-handed sites is located within this protein-coding sequence; a B-to-Z transition within this site may be involved in protein-IV gene regulation in vivo.
Similar content being viewed by others
References
Miller FD, Jorgenson KF, Winkfein RJ, van de Sande JH, Zarling DA, Stockton J & Rattner JB (1983) J. Biomolec. Struct. Dynamics1, 611–620.
Stockton JF, Miller FD, Jorgenson KF, Zarling DA, Morgan AR, Rattner JB & van de Sande JH (1983) EMBO J.2, 2123–2128.
Jovin TM, McIntosh LP, Arndt-Jovin DJ, Zarling DA, Robert-Nicoud M, van de Sande JH, Jorgenson KF & Eckstein F (1983) J. Biomolec. Struct. Dynamics1, 21–67.
Peck LJ, Nordheim A, Rich A & Wang JC (1982) Proc. Natl. Acad. Sci. U.S.A.79, 4560–4564.
Wells RD, Brennan R, Chapman KA, Goodman TC, Hart PA, Hillen W, Kellogg DR, Kilpatrick MW, Klein RD, Klysik J, Lambert PR, Larson JE, Miglietta JJ, Neuendorf SK, O'Connor TR, Singleton C, Stirdivant SM, Veneziale CM, Wartell RM & Zacharias W (1982) Cold Spring Harbor Symp. Quant. Biol.47, 77–84.
Haniford DB & Pulleyblank DE (1983) Nature (London)302, 632–634.
Nordheim A & Rich A (1983) Proc. Natl. Acad. Sci. U.S.A.80, 1821–1825.
Nordheim A, Lafer Em, Peck LJ, Wang JC, Stollar BD & Rich A (1982) Cell31, 309–318.
Azorin F, Nordheim A & Rich A (1983) EMBO J.2, 649–655.
Dicapua E, Stasiak A, Koller TH, Brahms S, Thomae R & Pohl FM (1983) EMBO J.2, 1531–1532.
Nordheim A & Rich A (1983) Nature (London)303, 674–679.
Thomae R, Beck S & Pohl FM (1983) Proc. Natl. Acad. Sci. U.S.A.80, 5550–5553.
Aiken JM, Miller FD, Hagen F, McKenzie DE, van de Sande JH & Dixon GH (1984) Proc. Natl. Acad. Sci. U.S.A. (submitted).
Espejo RT & Canelo ES (1968) Virology34, 738–747.
Lee JS & Morgan AR (1978) Can. J. Biochem.56, 585–591.
Kowalski D (1979) Anal. Biochem.93, 346–354.
Panet A, van de Sande JH, Loewen PE, Khorana HG, Raae AJ, Lillehaug JR & Kleppe K (1973) Biochemistry12, 5045–5050.
Zarling DA, Arndt-Jovin DJ, Robert-Nicoud M, McIntosh LP, Thomae R & Jovin TM (1984) J. Mol Biol., in press.
Maxam A & Gilbert W (1980) Meth. Enzym.65, 499–560.
Southern E (1975) J. Mol. Biol.98, 503–517.
Rigby PWJ, Dieckmann M, Rhodes C & Berg P (1977) J. Mol. Biol.113, 237–251.
Brewer GJ & Singh M (1982) J. Gen. Virol.60, 135–146.
Brewer GJ (1979) J. Virol.30, 875–882.
Hinnen R, Chassin R, Schafer R, Franklin R, Hitz H & Schafer D (1976) Eur. J. Biochem.68, 139–152.
Satake H, Kania M & Franklin RM (1981) Eur. J. Biochem.114, 623–628.
Peck L & Wang JC (unpublished results).
Durand R, Job C, Zarling DA, Teissere M, Jovin TM & Job D (1983) EMBO J.2, 1707–1714.
van de Sande JH & Jovin TM (1982) EMBO J.1, 115–120.
Espejo RT, Canelo ES & Sinsheimer RL (1981) J. Mol. Biol.56, 597–621.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Miller, F.D., Winkfein, R.J., Rattner, J.B. et al. Sequence analysis of a PM2-DNA anti-Z-IgG-binding region. Biosci Rep 4, 885–895 (1984). https://doi.org/10.1007/BF01138171
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01138171