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Up-modulation of phorbol dibutyrate receptors by carbachol and arachidonic acid in rat prostatic epithelial cells

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Bioscience Reports

Abstract

Phorbol dibutyrate (PDBu) binding to rat prostatic epithelial cells has been measured as an indirect determination of protein kinase C in this cell system. Analysis of [3H]PDBu binding using competitive displacement demonstrated a single class of PDBu receptors with a Kd=141 nM and a binding capacity of 4.8 pmol PDBu bound/mg cell protein. Raising cytosolic Ca2+ levels by redistribution of intracellular Ca2+ after cell treatment with carbachol or arachidonic acid (which also affects the bulk biophysical properties of the cell membrane) resulted in up-regulation of the available number of PDBu receptors. These results appear to be a consequence of PKC translocation from the cytosolic compartment to the plasma membrane after a cytosolic Ca2+ increase, confirming previous results in other cell systems.

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Authors and Affiliations

  1. Unidad de Neuroendocrinología Molecular, Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, 28871, Alcalá de Henares-Madrid, Spain

    J. C. Prieto

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  1. M. P. García-Paramio
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  2. M. J. Carmena
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  3. J. C. Prieto
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García-Paramio, M.P., Carmena, M.J. & Prieto, J.C. Up-modulation of phorbol dibutyrate receptors by carbachol and arachidonic acid in rat prostatic epithelial cells. Biosci Rep 11, 189–194 (1991). https://doi.org/10.1007/BF01136852

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  • DOI: https://doi.org/10.1007/BF01136852

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