Summary
Root-colonizingPseudomonas putida WCS358 enhances growth of potato in part by producing under iron-limiting conditions a yellow-green, fluorescent siderophore designated pseudobactin 358. This siderophore efficiently complexes iron(III) in the rhizosphere, making it less available to certain endemic microorganisms, including phytopathogens, thus inhibiting their growth. At least 15 genes distributed over five gene clusters are required for the biosynthesis of pseudobactin 358. High-affinity iron(III) transport in strain WCS358 is initiated by an 86-kDa outer membrane receptor protein (PupA) which appears to be specific for ferric pseudobactin 358. PupA shares strong similarity with TonB-dependent receptor proteins ofEscherichia coli, which suggests a TonB-like protein in strain WCS358 is required for iron(III) transport. Strain WCS358 possesses a second uptake system for ferric pseudobactin 358 and structurally diverse ferric siderophores produced by other microorganisms. A second receptor gene (pupB) responsible for iron transport from pseudobactin BN7 or pseudobactin BN8 has been identified. The production of this and certain other ferric siderophore receptor proteins requires that strain WCS358 be grown in the presence of these siderophores. An apparent regulatory gene required for the expression ofpupB is located adjacent topupB. Two positive regulatory genes have been identified which can independently activate, under low-iron(III) conditions, transcription of genes coding for the biosynthesis of pseudobactin 358.
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Bitter W, Marugg JD, de Weger LA, Tommassen J, Weisbeek PJ (1991) The ferric-pseudobactin receptor PupA ofPseudomonas putida WCS358: homology to TonB-dependentEscherichia coli receptors and specificity of the protein. Mol Microbiol (in press)
Briskot G, Taraz K, Budzikiewicz H (1989) Pyoverdin-type siderophores fromPseudomonas aeruginosa. Liebigs Ann Chem 1989:375–384
Burr TJ, Caesor AJ (1984) Beneficial plant bacteria. CRC Crit Rev Plant Sci 2:1–20
Coulton JW, Mason P, Cameron DR, Carmel G, Jean R, Rode HN (1986) Protein fusions ofβ-galactosidase to the ferrichrome-iron receptor ofEscherichia coli K12. J Bacteriol 165:181–192
Hantke K, Braun V (1975) Functional interaction of thetonA/tonB receptor system inEscherichia coli. J Bacteriol 135:190–197
Heller K, Kadner RJ (1985) Nucleotide sequence of the gene for the vitamin B12 receptor protein in the outer membrane ofEscherichia coli. J Bacteriol 161:904–908
Kloepper JW, Lifshiftz R, Zablotowicz RM (1989) Free-living bacterial inocula for enhancing crop productivity. Trends Biotechnol 6:39–44
Krone WJA, Stegehuis F, Koningstein G, van Doorn C, Roosendaal B, de Graaf FK, Oudega B (1985) Characterization of the pCo1V-K30-encoded cloacin DF13/aerobactin outer membrane receptor protein ofEscherichia coli; isolation and purification of the protein and analysis of its nucleotide sequence and primary structure. FEMS Microbiol Lett 26:153–161
Leong J (1986) Siderophores: their biochemistry and possible role in the biocontrol of plant pathogens. Annu Rev Phytopathol 24:187–209
Lundrigan MD, Kadner RJ (1986) Nucleotide sequence of the gene for the ferrienterochelin receptor FepA inEscherichia coli. J Biol Chem 261:10797–10801
Magazin M, Moores JC, Leong J (1986) Cloning of the gene coding for the outer membrane receptor protein for ferric pseudobactin, a siderophore from a plant growth-promotingPseudomonasstrain. J Biol Chem 261:795–799
Marugg JD, Nielander HB, Horrevoets AJG, van Megen I, van Genderen I, Weisbeek PJ (1988) Genetic organization and transcriptional analysis of a major gene cluster involved in siderophore biosynthesis inPseudomonas putida WCS358. J Bacteriol 170:1812–1819
Marugg JD, de Weger LA, Nielander HB, Oorthuizen M, Recourt K, Lugtenberg B, van der Hofstad GAJM, Weisbeek PJ (1989) Cloning and characterization of a gene encoding an outer membrane protein required for siderophore-mediated uptake of Fe3+ inPseudomonas putida WCS358. J Bacteriol 171:2819–2826
Moores JC, Magazin M, Ditta GS, Leong J (1984) Cloning of genes involved in the biosynthesis of pseudobactin, a highaffinity iron transport agent of a plant growth-promotingPseudomonas strain. J Bacteriol 157:53–58
Nau CD, Konisky J (1989) Evolutionary relationship between the TonB-dependent outer membrane transport proteins: nucleotide and amino acid sequences of theEscherichia coli colicin I receptor gene. J Bacteriol 171:1041–1047
Persmark M, Frejd T, Mattiasson B (1990) Purification, characterization, and structure of pseudobactin 589A, a siderophore from a plant growth promotingPseudomonas. Biochemistry 29:7348–7356
Pressler U, Staudenmaier H, Zimmermann L, Braun V (1988) Genetics of the iron dicitrate transport system ofEscherichia coli. J Bacteriol 170:2716–2724
Salinas PC, Tolmasky ME, Crosa JH (1989) Regulation of the iron uptake system inVibrio anguillarum: evidence for a cooperative effect between two transcriptional activators. Proc Natl Acad Sci USA 86:3529–3533
Sauer K, Hantke K, Braun V (1990) Sequence of thefhuE outer membrane receptor gene ofEscherichia coli K12 and properties of mutants. Mol Microbiol 4:427–437
Schippers B, Bakker AW, Bakker PAHM (1987) Interactions of deleterious and beneficial rhizosphere microorganisms and the effect of cropping practices. Annu Rev Phytopathol 25:339–358
Teintze M, Hossain MB, Barnes CL, Leong J, van der Helm D (1981) Structure of ferric pseudobactin, a siderophore from a plant growth-promotingPseudomonas. Biochemistry 20:6446–6457
Tommassen J (1988) Biogenesis and membrane topology of outer membrane proteins inEscherichia coli. In: op den Kamp JAF (ed) Membrane biogenesis, Springer Verlag, Berlin, Heidelberg, pp 351–373
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Leong, J., Bitter, W., Koster, M. et al. Molecular analysis of iron transport in plant growth-promotingPseudomonas putida WCS358. Biol Metals 4, 36–40 (1991). https://doi.org/10.1007/BF01135555
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DOI: https://doi.org/10.1007/BF01135555