Summary
An NADH-dependent ferric reductase was identified in extracts ofNeisseria gonorrhoeae. Enzyme activity was measured in an assay using ferrozine as the ferrous iron acceptor. Ferric reductase activity was enhanced by Mg2+ and flavine nucleotides. The enzyme reduced both citrate- and diphosphate-bound ferric iron as well as ferric hydroxide (Imferon). However, no activity was observed with either 30%-iron-saturated transferrin or with the gonococcal iron-binding protein, Fbp. The ferric reductase was found primarily within the cytoplasmic cell fraction. The soluble ferric reductase was purified 110-fold by ammonium sulfate precipitation, gel and anion-exchange chromatography. Results obtained following gel chromatography and SDS/polyacrylamide gel electrophoresis suggested that the enzyme had a molecular mass of about 25 kDa.
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Le Faou, A.E., Morse, S.A. Characterization of a soluble ferric reductase fromNeisseria gonorrhoeae . Biol Metals 4, 126–131 (1991). https://doi.org/10.1007/BF01135390
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DOI: https://doi.org/10.1007/BF01135390