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Human pregnancy by in vitro fertilization (IVF) using sperm aspirated from the epididymis

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Spermatozoa were collected by microaspiration from the corpus epididymidis of a 42-year-old man with secondary obstructive azoospermia and used for in vitro fertilization. At insemination 61% of the spermatozoa were motile, with a motility index of 157. One of five eggs was fertilized and this was subsequently transferred to the patient's wife at the two-cell stage. Ultrasound examination and changing hormone levels confirmed an ongoing pregnancy, which is currently at 30 weeks of gestation. This technique will provide a useful alternative for the management of some infertile men with obstructive azoospermia.

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Temple-Smith, P.D., Southwick, G.J., Yates, C.A. et al. Human pregnancy by in vitro fertilization (IVF) using sperm aspirated from the epididymis. J Assist Reprod Genet 2, 119–122 (1985).

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