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Levels of five mycotoxins in grains harvested in Atlantic Canada as measured by high performance liquid chromatography

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Abstract

An analytical protocol using high performance liquid chromatography (HPLC) was used to analyze samples of spring wheat, winter wheat, 2-row barley, and 6-row barley over a period of three years for the presence of five mycotoxins. These included deoxynivalenol, zearalenone, T-2 toxin, HT-2 toxin, and diacetoxyscirpenol. The protocol employed a single extraction step using acetonitrile-water and two cleanup procedures. One utilized a solid-phase extraction column and the other a charcoal-alumina column. Detection limits ranged from 0.02 to 0.15 μg of mycotoxin g−1 grain. Little T-2 toxin, HT-2 toxin, or diacetoxyscirpenol was found in the samples. Deoxynivale nol was detected in 53 to 62% of the samples tested and zearalenone in 25–29% of the samples. Several enzyme-linked immunosorbent assays were used for comparison purposes with deoxynivalenol, zearalenone, and T-2 toxin. These kits provided reliable qualitative, but not quantitative, data.

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References

  • Arpad B, Andras V, Lasztity R (1983) Simultaneous detection of some fusariotoxins by gas-liquid chromatography. J Assoc Offic Anal Chem 66:577–581

    Google Scholar 

  • Bagneris RW, Gaul JA, Ware GM (1986) Liquid chromatographic determination of zearalenone and zearalenol in animal feeds and grains using fluorescence detection. J Assoc Offic Anal Chem 69:894–898

    Google Scholar 

  • Beasley VR, Bunner DL, Poppenga RH (1989) Conclusions and future directions for research. In: Beasley VR (ed) Trichothecene mycotoxicosis: Pathophysiologic effects. CRC Press, Boca Raton, FL, pp 169–189

    Google Scholar 

  • Betina V (1989) Chromatographic methods as tools in the field of mycotoxins. J Chromatogr 477:187–233

    Google Scholar 

  • Chamkasem N, Cobb WY, Latimer GW, Salinas C, Clement BA (1989) Liquid chromatographic determination of aflatoxins, ochratoxin A, and zearalenone in grains, oilseeds, and animal feeds by post-column derivatization and on-line sample cleanup. J Assoc Offic Anal Chem 72:336–341

    Google Scholar 

  • Chang HL, DeVries JW (1984) Short liquid chromatographic method for the determination of zearalenone and alpha-zearalenone. J Assoc Offic Anal Chem 67:741–744

    Google Scholar 

  • Childress WL, Krull IS, Selavka CM (1990) Determination of deoxynivalenol (DON, Vomitoxin) in wheat by high-performance liquid chromatography with photolysis and electrochemical detection (HPLC-hv-EC). J Chromatogr Sci 28:76–82

    Google Scholar 

  • Cohen H, LaPointe M (1982) Capillary gas-liquid chromatographic determination of vomitoxin in cereal grains. J Assoc Offic Anal Chem 65: 1429–1434. Correction in J Assoc Off Anal Chem 66:821

    Google Scholar 

  • Dixon DE, Warner RL, Ram BP, Hart LP, Pestka JJ (1987) Hybridoma cell line production of a specific monoclonal antibody to the mycotoxins zearalenone and alpha-zearalenone. J Agric Food Chem 35:122–126

    Google Scholar 

  • Fowlie P (1990) Detection limit what does it mean? Wastewater Technol Centre Newsletter No 17, Sept 1990 Environment Canada, Burlington, ON, pp 7–8

    Google Scholar 

  • Francis BM (1989) Reproductive toxicology of trichothecenes. In: Beasley VR (ed) Trichothecene mycotoxicosis: Pathophysiologic effects. CRC Press, Boca Raton, FL, pp 143–159

    Google Scholar 

  • Genloff EH, Pestka JJ, Swanson SP, Hart LP (1984) Detection of T-2 toxin in Fusarium sporotrichiodes-infected corn by enzyme-linked immunosorbent assay. Appl Environ Microbiol 47:1161–1163

    Google Scholar 

  • Genloff EH, Pestka JJ, Dixon DE, Hart LP (1987) Production of a monoclonal antibody to T-2 toxin with strong cross-reactivity to T-2 metabolites. Phytopathology 77:57–59

    Google Scholar 

  • Jelinek CF, Pohland AE, Wood GE (1989) Worldwide occurrence of mycotoxins in foods and feeds—an update. J Assoc Offic Anal Chem 72:223–230

    Google Scholar 

  • Kamimura H, Nishijima M, Yasuda K, Saito K, Ibe A, Nagayama T, Ushiyama H, Naoi Y (1981) Simultaneous detection of several mycotoxins in cereals, grains and foodstuffs. J Assoc Offic Anal Chem 64:1067–1073

    Google Scholar 

  • Kuronen P (1989) High-performance liquid chromatography screening method for mycotoxins using new retention indexes and diode array detection. Arch Environ Contain Toxicol 18:336–348

    Google Scholar 

  • Lauren DR, Greenhalgh R (1987) Simultaneous analysis of nivalenol and deoxynivalenol in cereals by liquid chromatography. J Assoc Offic Anal Chem 70:479–483

    Google Scholar 

  • Lee US, Jang HS, Tanaka T, Toyasaki N, Sugiura Y, Oh YJ, Cho CM, Ueno Y (1986) Mycological survey of Korean cereals and production of mycotoxins byFusarium isolates. Appl Environ Microbial 53:1258–1260

    Google Scholar 

  • Pestka JJ (1988) Enhanced surveillance of foodborne mycotoxins by immunochemical assay. J Assoc Offic Anal Chem 71:1075–1081.

    Google Scholar 

  • Rajakyla E, Laasasenaho K, Sakkers PJD (1987) Determination of mycotoxins in grain by high performance liquid chromatography and thermospray liquid chromatography-mass spectrometry. J Chromatogr 384:391–402

    Google Scholar 

  • Romer TR (1986) Use of small charcoal/alumina cleanup columns in the determination of trichothecene mycotoxins in foods and feeds. J Assoc Offic Anal Chem 69:699–703

    Google Scholar 

  • Rood HD, Buck WB, Swanson SP (1988) Gas chromatographic screening method for T-2 toxin, diacetoxyscirpenol, deoxynivalenol, and related trichothecenes in feeds. J Assoc Offic Anal Chem 71:493–498

    Google Scholar 

  • Rotter BA, Thompson BK, Prelusky DB, Trenholm HL (1991) Evaluation of potential interactions involving trichothecene mycotoxins using the chick embryotoxicity bioassay. Arch Environ Contam Toxicol 21:621–624

    Google Scholar 

  • Scott PM (1982) Assessment of quantitative methods for determination of trichothecenes in grains and grain products. J Assoc Offic Anal Chem 65:876–883

    Google Scholar 

  • — (1989) The natural occurrence of trichothecenes. In: Beasley VR (ed) Trichothecene mycotoxicosis: Pathophysiologic effects. CRC Press, Boca Raton, FL, pp 1–26

    Google Scholar 

  • Scott PM, Kanhere SR, Tarter EJ (1986) Determination of nivalenol and deoxynivalenol in cereals by election-capture gas chromatography. J Assoc Offic Anal Chem 69:889–893

    Google Scholar 

  • Scott PM, Lombaert GA, Pellaers P, Bacler S, Kanhere SR, Sun WF, Lau PY, Weber D (1989) Application of capillary gas chromatography to a survey of wheat for five trichothecenes. Food Additives Contam 6:489–500

    Google Scholar 

  • Seaman WL (1982) Epidemiology and control of mycotoxigenic fusaria on cereal grains. Can J Plant Pathol 4:187–190

    Google Scholar 

  • Shannon GM, Peterson RE, Shotwell OL (1985) Rapid screening method for detection of deoxynivalenol. J Assoc Offic Anal Chem 68:1126–1128

    Google Scholar 

  • Siame BA, Lovelace CEA (1989) Natural occurrence of zearalenone and trichothecene toxins in maize-based animal feeds in Zambia. J Agric Food Sci 49:25–35

    Google Scholar 

  • Terhune SJ, Nguyen NV, Baxter JA, Pryde DH, Qureshi SA (1984) Improved gas chromatographic method for quantitation of deoxynivalenol in wheat, corn, and feed. J Assoc Offic Anal Chem 67:402–404

    Google Scholar 

  • Trenholm HL, Warner RM, Prelusky DB (1985) Assessment of extraction procedures in the analysis of naturally contaminated grain products for deoxynivalenol (vomitoxin). J Assoc Offic Anal Chem 68:645–649

    Google Scholar 

  • Trenholm HL, Prelusky DB, Young JC, Miller JD (1989) A practical guide to the prevention ofFusarium mycotoxins in grain and animal feedstuffs. Arch Environ Contamin Toxicol 18:443–451

    Google Scholar 

  • Tutelyan VA, Eller KI, Sobolev SS, Pimenova VV, Zakharova LP, Muzychenko NI (1990) A survey of the occurrence of deoxynivalenol in wheat from 1986–1988 harvests in the USSR. Food Additives Contam 7:521–525

    Google Scholar 

  • Uneo Y (1983) In: Uneo Y (ed) Trichothecenes: Chemical, biological, and toxicological aspects. Elsevier Science Publishing Inc, NY, pp 195–301

    Google Scholar 

  • Ware G, Thorpe C (1979) Abstr 93rd Ann Mtg J Assoc Offic Anal Chem Oct 15–18, 1979, p 74

  • Ware GM, Francis OJ, Carman AS, Kuan SS (1986) Gas chromatographic determination of deoxynivalenol in wheat with electron capture detection: Collaborative study. J Assoc Offic Anal Chem 69:899–901

    Google Scholar 

  • Warner R, Ram BP, Hart LP, Pestka JJ (1986) Screening for zearalenone in corn by competitive direct enzyme-linked immunosorbent assay. J Agric Food Chem 34:714–717

    Google Scholar 

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Authors and Affiliations

  1. Department of Biology, P. O. Box 550, B2N 5E3, Truro, Nova Scotia, Canada

    G. W. Stratton

  2. Department of Chemistry and Soil Science, Nova Scotia Agricultural College, P. 0. Box 550, B2N 5E3, Truro, Nova Scotia, Canada

    A. R. Robinson & H. C. Smith

  3. Nova Scotia Department of Agriculture and Marketing, P. O. Box 550, B2N 5E3, Truro, Nova Scotia, Canada

    L. Kittilsen & M. Barbour

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  1. G. W. Stratton
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  2. A. R. Robinson
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  3. H. C. Smith
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  4. L. Kittilsen
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  5. M. Barbour
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Stratton, G.W., Robinson, A.R., Smith, H.C. et al. Levels of five mycotoxins in grains harvested in Atlantic Canada as measured by high performance liquid chromatography. Arch. Environ. Contam. Toxicol. 24, 399–409 (1993). https://doi.org/10.1007/BF01128740

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  • DOI: https://doi.org/10.1007/BF01128740

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