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Intestinal absorption of biotin and biocytin in the rat

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Abstract

The uptake of biotin and biocytin was investigated in rat intestine using the everted sac technique. It has been shown that at biotin and biocytin concentrations !ess than 40 and 50 nM respectively, absorption proceeds by a saturable process, whereas at higher concentrations uptake by passive diffusion predominates. Fractionation of solublized brush border preparations indicates that biotinidase is the only protein which binds biotin in this preparation.

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Authors and Affiliations

  1. Department of Biochemistry, University of Manitoba, R3E 0W3, Winnipeg, Canada

    K. Dakshinamurti, J. Chauhan & H. Ebrahim

Authors
  1. K. Dakshinamurti
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  2. J. Chauhan
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  3. H. Ebrahim
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Dakshinamurti, K., Chauhan, J. & Ebrahim, H. Intestinal absorption of biotin and biocytin in the rat. Biosci Rep 7, 667–673 (1987). https://doi.org/10.1007/BF01127680

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  • DOI: https://doi.org/10.1007/BF01127680

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