Skip to main content
SpringerLink
Log in

Spectrometric investigations of the energy system of living neurons

  • Published:
Neuroscience Translations Aims and scope Submit manuscript

Summary

  1. 1.

    The cytochrome c — cytochrome oxidase system in molluscan neurons is located in narrow (5–7 μ) zones near the membrane in high concentration and evidently supplies the whole of the energy required for active transport of ions through the membrane.

  2. 2.

    Places of synthesis of mitochondria are characterized by extintion spectra containing absorption bands of enzymes of the cytochrome c — cytochrome oxidase system and the cytochrome b5 system simultaneously.

  3. 3.

    It is shown that the method of microspectrometry of living cells provides an approach to the study of kinetics of intracellular processes and can be used to test the functional state of the cell itself and of its organoids with respect to the ratio between oxidized and reduced forms of cytochrome in the terminal oxidation chain.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. IONETANI, T. J. Biol. Chem. 236:845, 1960.

    Google Scholar 

  2. GREEN, D. E., J. JARNEFELT and H. D. TISDALE. Biochim. Biophys. Acta 31:34, 1959.

    Google Scholar 

  3. BOMSTEIN, R., R. GOLDENBERGER and H. D. TISDALE. Biochem. Biophys. Res. Commun. 3:479, 1960.

    Google Scholar 

  4. GOLDENBERGER, R., A. L. SMITH, H. D. TISDALE and R. BOMSTEIN. J. Biol. Chem. 236:2788, 1961.

    Google Scholar 

  5. MARGOLIASH, E. and N. FROHWIRT. Biochem. J. 71:570, 1959.

    Google Scholar 

  6. KEILIN, D. and E. SLATER. Brit. Med. Bull. 9:89, 1953.

    Google Scholar 

  7. STRITTMATTER, P. and S. F. VELICK. J. Biol. Chem. 221:253, 1956.

    Google Scholar 

  8. STRITTMATTER, P. J. Biol. Chem. 235:2492, 1960.

    Google Scholar 

  9. STRITTMATTER, P. J. Biol. Chem. 236:2329, 1961.

    Google Scholar 

  10. STRITTMATTER, P. and S. F. VELICK. J. Biol. Chem. 228:785, 1957.

    Google Scholar 

  11. KEILIN, D. Biochem. J. 64:663, 1956.

    Google Scholar 

  12. THORELL, B. and B. CHANCE Exp. Cell Res. 20:43, 1960.

    Google Scholar 

  13. KARNAUKHOV, V. N., S. I. ROZANOV and V. A. SVOREN'. Biofizika. 11:1085, 1966.

    Google Scholar 

  14. CASPERSON, T. Skand. Arch. Physiol. 73, Suppl. 8:1, 1936.

    Google Scholar 

  15. POLLISTER, A. and L. ORNSTEIN. Methods of Cytologic Analysis. Foreign Literature Press, Moscow, 1957, p. 9.

    Google Scholar 

  16. AGROSKIN, L. S., V. Ya. BRODSKII, A. D. GRUZDEV and V. N. KOROLEV. Tsitologiya. 2:337, 1960.

    Google Scholar 

  17. BELL, L. N. Biofizika, 10:374, 1965.

    Google Scholar 

  18. JAKOBS, E. and S. HOLT. J. Chem. Phys. 22:142, 1954.

    Google Scholar 

  19. DE DUVE, C. and J. BERTHET. Int. Rev. Cytol. 3:225, 1954.

    Google Scholar 

  20. LEVIN, S. V. Tsitologiya. 2(4):1960.

  21. SAKHAROV, D. A.. Usp. Sovrem. Biol. 60:365,1965.

    Google Scholar 

  22. REDFIELD, A. S. Usp. Sovrem. Biol. 5:902, 1963.

    Google Scholar 

  23. ROSKIN, G. I., and L. B. LEVINSON. Microscopic Techniques. GISN, Moscow, 1957, pp. 74–75.

    Google Scholar 

  24. CHANCE, B., R. PERRY, L. AKKERMAN and B. THORELL. Rev. Sci. Instrum. 30:735, 1959.

    Google Scholar 

  25. AMOROSO, E. C., M. J. BAXTER, A. D. CHIQUIONE and R. H. NISBERT. Proc. Roy. Soc. 160B:979, 167, 1964.

    Google Scholar 

  26. TRUJIOLLO-CENOZ, O. Z. Zellforsch. 56:649, 1962.

    Google Scholar 

  27. ROSENBLUTH, J. Z. Zellforsch. 60:213, 1963.

    Google Scholar 

  28. ARVANITAKI, A. and N. CHALAZONITIS. Arch. Sci. Physiol. 12:2, 1958.

    Google Scholar 

  29. ARVANITAKI, A. and N. CHALAZONITIS. Arch. Sci. Physiol. 111(1): 1949.

  30. VERZHBINSKAYA, N. A. Material on Evolutionary Physiology, Vol. 1, 1956, p. 59.

    Google Scholar 

  31. KING, T. E. Proceedings of the 5th International Biochemical Congress, Symposium 5. USSR Acad. Sci. Press, Moscow, 1962, p. 411.

    Google Scholar 

  32. SCHARF, J. H. and R. BLUME. Z. Zellforsch. 62:454 and 467, 1964.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biological Physics, USSR Academy of Sciences, Pushchino-on-the Oka

    V. N. Karnaukhov

Authors
  1. V. N. Karnaukhov
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Karnaukhov, V.N. Spectrometric investigations of the energy system of living neurons. Neurosci Behav Physiol 2, 876–884 (1968). https://doi.org/10.1007/BF01124735

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01124735

Keywords

Search

Navigation