Skip to main content
SpringerLink
Log in

Studies on the subcellular localization and role of glutathione-insulin transhydrogenase in rat liver

  • Published:
Bioscience Reports

Abstract

125I-insulin was shown to be internalized in vivo to a discrete population of low-density membranes (ligandosomes), distinct from the Golgi, endoplasmic reticulum, plasma membrane, and lysosomes. However, analytical subcellular fractionation shows that glutathione-insulin transhydrogenase is localized to the endoplasmic reticulum. Measurement of the specific enzyme activity of glutathione-insulin transhydrogenase showed no differences between normal, diabetic, and hyperinsulinaemic rats. These results suggest that glutathione-insulin transhydrogenase is not directly involved in the subceltular processing of receptor-bound internalized insulin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Varandani PT (1973) Biochim. Biophys. Acta304, 642–659.

    Google Scholar 

  2. Uete T, Shimano N, Shimizu S & Morikawa M (1976) Metabolism25, 4, 375–384.

    Google Scholar 

  3. Thomas JH & Varandani PT (1979) Biochim. Biophys. Acta567, 88–95.

    Google Scholar 

  4. Thomas JH & Wakefield SM (1973) Biochem. Soc. Trans.1, 1179–1182.

    Google Scholar 

  5. Amar-Costesec A, Wibo M, Thines-Sempoux D, Beaufay H & Bethet J (1974) J. Cell Biol.62, 717–745.

    Google Scholar 

  6. Varandani PT, Raveed D & Nafz MA (1978) Biochim. Biophys. Acta538, 343–353.

    Google Scholar 

  7. Smith GD & Peters TJ (1980) Eur. J. Biochem.104, 305–311.

    Google Scholar 

  8. Smith GD, Evans WH & Peters TJ (1980) FEBS Lett.120, 104–106.

    Google Scholar 

  9. Jorgensen KH & Larsen UD (1980) Diabetologia19, 546–554.

    Google Scholar 

  10. Smith GD & Peters TJ (1982) Biochim. Biophys. Acta716, 24–30.

    Google Scholar 

  11. Manchester KL (1970) in ‘Biochemical Action of Hormones’, vol 1 (G. Litwack, ed), pp 267–320, Academic Press.

Download references

Author information

Authors and Affiliations

  1. Division of Clinical Cell Biology, MRC Clinical Research Centre, Watford Road, Harrow, Middlesex

    Balvinder K. Chowdhary, Geoffrey D. Smith & Timothy J. Peters

  2. Clinical Sciences, MRC Clinical Research Centre, Watford Road, Harrow, Middlesex

    Robert Mahler

Authors
  1. Balvinder K. Chowdhary
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Geoffrey D. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Robert Mahler
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Timothy J. Peters
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chowdhary, B.K., Smith, G.D., Mahler, R. et al. Studies on the subcellular localization and role of glutathione-insulin transhydrogenase in rat liver. Biosci Rep 3, 323–329 (1983). https://doi.org/10.1007/BF01122896

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01122896

Keywords

Search

Navigation