Skip to main content
SpringerLink
Log in

Vergleichende Untersuchungen der trypsinspezifischen reaktiven Zentren verschiedener Phaseolus coccineus- und Phaseolus vulgaris-Inhibitoren

Comparative studies on the reactive sites against trypsin of some inhibitors from Phaseolus coccineus and Phaseolus vulgaris

  • Published:
Zeitschrift für Lebensmittel-Untersuchung und Forschung Aims and scope Submit manuscript

Summary

Three of the trypsin chymotrypsin inhibitors from the seeds of runner beans (Phaseolus coccineus L.), PCI 3, 42, and 5, and three of the inhibitors from the seeds of french beans (Phaseolus vulgaris var. nanus), PVI 3, 4, and 5, contain a lysine residue in the reactive site against trypsin. One of the inhibitors fromPhaseolus coccineus, PCI 2, contains an arginine residue there. All sevenPhaseolus inhibitors investigated are double headed.

Zusammenfassung

Drei Trypsin-Chymotrypsin-Inhibitoren aus den Samen von Feuerbohnen (Phaseolus coccineus L.) PCI 3, 42 und 5 und drei Inhibitoren aus den Samen von Buschbohnen (Phaseolus vulgaris var. nanus) PVI 3, 4 und 5 enthalten im reaktiven Zentrum gegen Trypsin einen Lysin-Rest. Ein Inhibitor ausPhaseolus coccineus, PCI 2, enthält dort einen Arginin-Rest. Alle sieben untersuchtenPhaseolus-Inhibitoren sinddouble headed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  1. Ortanderl,H.H., Belitz,H.-D.: Lebensm.-Wiss. Technol.4, 85 (1971)

    Google Scholar 

  2. Belitz,H.-D., Fuchs, A., Grimm, L.: Lebensm.-Wiss. Technol.4, 89 (1971)

    Google Scholar 

  3. Belitz,H.-D., Fuchs,A., Nitsche,G., Al-Sultan,T.: Z. Lebensm.-Unters.-Forsch.150, 215 (1972)

    Google Scholar 

  4. Weder,J.K.P.: Chem. Mikrobiol. Technol. Lebensm.3, 89 (1974)

    Google Scholar 

  5. Weder,J.K.P., Kassubek,A.: Z. Lebensm.-Unters.-Forsch.156, 22 (1974)

    Google Scholar 

  6. Weder,J.K.P., Kassubek,A., Hory,H.-D.: Chem. Mikrobiol. Technol. Lebensm.4, 79 (1975)

    Google Scholar 

  7. Gerstenberg, H., Belitz, H.-D., Weder, J.K.P.: in Vorbereitung

  8. Ozawa,K., Laskowski,M., jr.: J. Biol. Chem.241, 3955 (1966)

    PubMed  Google Scholar 

  9. Butler,P.J.G., Harris,J.I., Hartley,B.S., Leberman,R.: Biochem. J.112, 679 (1969)

    PubMed  Google Scholar 

  10. Haynes,R., Osuga,D.T., Feeney,R.E.: Biochemistry6, 541 (1967)

    PubMed  Google Scholar 

  11. Grossberg,A.L., Pressman,D.: Biochemistry7, 272 (1968)

    PubMed  Google Scholar 

  12. Means,G.E., Feeney,R.E.: Chemical modification of proteins, p.224. San Francisco: Holden-Day, Inc. 1971

    Google Scholar 

  13. Kaiser,K.-P., Belitz,H.-D.: Chem. Mikrobiol. Technol. Lebensm.1, 1 (1971)

    Google Scholar 

  14. Weder,J., Hory,H.-D.: Lebensm.-Wiss. Technol.5, 54 (1972)

    Google Scholar 

  15. Santarius,K., Belitz,H.-D.: Z. Lebensm.-Unters.-Forsch.154, 206 (1974)

    Google Scholar 

  16. Simlot,M.M., Feeney,R.E.: Arch. Biochem. Biophys.113, 64 (1966)

    PubMed  Google Scholar 

  17. Koshland,D.E., jr., Ray,W.J., jr., Erwin, M.J.: Fed. Proc.17, 1145 (1958)

    PubMed  Google Scholar 

  18. Weder,J.K.P., Hory,H.-D.: Chem. Mikrobiol. Technol. Lebensm.4, 161 (1976)

    Google Scholar 

  19. Laskowski,M.,jr., Sealock,R.W.: Protein proteinase inhibitors — Molecular aspects. In: Boyer,P.D. (Ed.): The Enzymes, Vol. III, p. 375. New York: Academic Press 1971

    Google Scholar 

  20. Wilson,K.A., Laskowski,M.,sr.: J. Biol. Chem.248, 756 (1973)

    PubMed  Google Scholar 

  21. Fritz,H., Fink,E., Gebhardt,M., Hochstrasser,K., Werle,E.: Hoppe-Seylers Z. Physiol. Chem.350, 933 (1969)

    PubMed  Google Scholar 

  22. Stevens,F.C., Wuerz,S., Krahn,J.: Structure-function relationships in lima bean protease inhibitor. In: Fritz,H., Tschesche,H., Greene,L.J., Truscheit,E. (eds.): Bayer-Symposium V: Proteinase Inhibitors, Grosse Ledder near Cologne, FRG, Oct. 16–20, 1973, p. 344. Berlin-Heidelberg-New York: Springer 1974

    Google Scholar 

  23. Birk,Y.: Structure-activity relationships of several trypsin and chymotrypsin inhibitors from legume seeds. In: Fritz,H., u. a., s. [22], p. 355

    Google Scholar 

  24. Horng,W.J., Gan,J.C.: Tex. Rep. Biol. Med.32, 489 (1974), zitiert nach CA82, 2492 (1975)

    PubMed  Google Scholar 

  25. Barrett,A.J., Starkey,P.M., Munn,E.A.: The unique nature of the interaction ofα 2-macroglobulin with proteinases. In: Fritz, H., u. a., s. [22], p. 72

    Google Scholar 

  26. Schiessler,H., Arnhold,M., Fritz,H.: Characterization of two proteinase inhibitors from human seminal plasma and spermatozoa. In: Fritz,H., u. a., s. [22], p. 147

    Google Scholar 

  27. Tschesche,H., Rücker,A., von: Discussion remark: Proteinase isoinhibitors from cuttle fish (Loligo vulgaris). In: Fritz,H., u. a., s. [22], Berlin-Heidelberg-New York: Springer 1974, p. 284

    Google Scholar 

  28. Belitz,H.-D., Fuchs,A.: Chem. Mikrobiol. Technol. Lebensm.1, 132 (1972)

    Google Scholar 

  29. Belitz,H.-D., Fuchs,A.: Z. Lebensm. Unters.-Forsch.152, 129 (1973)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Lebensmittelchemie der Technischen Universität München, Lothstr. 17, D-8000, München 2

    Heinz-Dieter Hory, Jürgen K. P. Weder & Hans-Dieter Belitz

Authors
  1. Heinz-Dieter Hory
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jürgen K. P. Weder
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hans-Dieter Belitz
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Die Arbeiten wurden durch die AIF über den Forschungskreis der Ernährungsindustrie gefördert

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hory, HD., Weder, J.K.P. & Belitz, HD. Vergleichende Untersuchungen der trypsinspezifischen reaktiven Zentren verschiedener Phaseolus coccineus- und Phaseolus vulgaris-Inhibitoren. Z Lebensm Unters Forch 162, 341–347 (1976). https://doi.org/10.1007/BF01122786

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01122786

Search

Navigation