Abstract
The intracellular and mitochondrial matrix water-permeable spaces were measured in intact, respiring hepatocytes from normal fed, 48 h starved, and protein fed rats. The mitochondrial matrix constitutes 15–20 % of the total intracellular water, a proportion greater than has generally been assumed.
References
Allard, C., de Lamirande, G. and Cantero, A. (1957).Exptl. Cell Res. 13:69–77.
Baur, H. and Heldt, H. W. (1977).Eur. J. Biochem. 74:397–403.
Beavis, A. D., Brannan, R. D. and Garlid, K. D. (1985).J. Biol. Chem. 260:13424–13433.
Blouin, A., Bolender, R. P. and Weibel, E. R. (1977).J. Cell. Biol. 72:441–455.
Candipan, R. C. and Sjöstrand, F. S. (1984a).J. Ultrastruct. Res. 89:249–260.
Candipan, R. C. and Sjöstrand, F. S. (1984b).J. Ultrastruct. Res. 89:281–294.
Cech, J. M., Freeman, R. B., Caro, J. F. and Amatruda, J. M. (1980).Biochem. J. 188:839–845.
Cohen, N. and Raijman, L. (1980).J. Biol. Chem. 255:3352–3357.
Cornell, N. W. (1980).Anal. Biochem. 1023:326–331.
Craik, J. D. and Elliott, K. R. F. (1979).Biochem. J. 182:503–508.
Didier, R., Remesy, C., Demigne, C. and Fafournoux, P. (1985).Nutr. Res. 5:1093–1102.
Farrell, R. and Lund, P. (1983).Biosci. Rep..3:539–543.
Foden, S. and Randle, P. J. (1978).Biochem. J. 170:615–625.
Greenawalt, J. W. (1974).Methods Enzymol. 31:310–323.
Hackenbrock, C. R. (1966).J. Cell. Biol. 30:269–297.
Halestrap, A. P. and Quinlan, P. T. (1983).Biochem. J. 214:387–393.
Harris, E. J. and Van Dam, K. (1968).Biochem. J. 106:759–766.
Krebs, H. A. and Henseleit, K. (1932).Hoppe-Seyler's Z. Physical Chem. 210:33–66.
Krebs, H. A., Cornell, N. W., Lund, P. and Hems, R. (1974). In:Regulation of Hepatic Metabolism (Lundquist, F. and Tygstrup, N., eds), pp. 726–750, Munksgaard, Copenhagen.
Loud, A. V. (1968).J. Cell. Biol. 37:27–46.
McNurlan, M. A., Tomkins, A. M. and Garlick, P. J. (1979).Biochem. J. 178:373–379.
Otto, D. A., Cook, G. A. and Reiss, P. D. (1983). In:Isolation Characterization, and Use of Hepatocytes (Harris, R. A. and Cornell, N. W., eds), pp. 41–48, Elsevier Science Publishing Co.
Pfaff, E., Klingenberg, M., Ritt, E. and Vogell, W. (1968).Eur. J. Biochem. 5:222–232.
Quinlan, P. T., Thomas, A. P., Armston, A. E. and Halestrap, A. P. (1983).Biochem. J. 214:395–404.
Rohr, H. P., Lüthy, J., Gudat, F., Oberholzer, M., Gysin, C., Stalder, G. and Bianchi, L. (1976).Prog. Liver Dis. V:24–34.
Sainsbury, G. M., Stubbs, M., Hems, R. and Krebs, H. A. (1979).Biochem. J. 180:685–688.
Scholz, R. and Bücher, Th. (1965). In:Control of Energy Metabolism (Chance, B., Estabrook, R. W. and Williamson, J. R., ed.), pp. 393–414, Academic Press, New York.
Soboll, S., Scholz, R., Freisl, M., Elbers, R. and Heldt, H. W. (1976). In:Use of Isolated Liver Cells and Kidney Tubules in Metabolic Studies (Tager, J. M., Söling, H. D. and Williamson, J. E., ed.), pp. 29–40, North-Holland Publishing Co., Amsterdam.
Tischler, M. E., Hecht, P. and Williamson, J. R. (1977).Arch. Biochem. Biophys. 181: 278–292.
Uhal, B. D. and Roehrig, K. L. (1982).Biosci. Rep. 2:1003–1007.
Volman, H. (1968).Virch. Arch. (Cell Pathol.) 26:249–259.
Weibel, E. R., Stäubli, W., Gnägi, H. R. and Hess, F. (1969).J. Cell. Biol. 42:68–91.
Zuurendonk, P. F. and Tager, J. M. (1974).Biochim. Biophys. Acta 333:393–399.
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Lund, P., Wiggins, D. The matrix water space of mitochondriain situ in isolated hepatocytes. Biosci Rep 7, 59–66 (1987). https://doi.org/10.1007/BF01122728
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DOI: https://doi.org/10.1007/BF01122728