Abstract
Native bovine parathyroid hormone (bPTH) was found to be readily cleaved with human leukocyte elastase to yield the fragments bPTH(1–41) and bPTH(42–84). These were then isolated by reverse-phase HPLC and characterised by gas-phase sequencing and amino acid analysis. The biological activities of these fragments were assessed in an adenylate cyclase bioassay using the rat osteosarcoma cell line UMR106. bPTH(1–41) was found to have approximately twice the molar potency of the native hormone from which it was derived, bPTH(42–84) had no biological activity and did not modulate the adenylate cyclase response to these cells to the native hormone. The possible physiological significance of these observations is discussed.
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References
Arber, C. E., Zanelli, J. M., Parsons, J. A., Bitensky, L. and Chayen, J. (1980).J. Endocrinol. 85:55P-56P.
Aurbach, G. D. (1959).J. Biol. Chem. 234:3179–3181.
Barling, P. M., Cartmell, K. and Scott, G. K. (1984).Int. J. Biochem. 16:815–821.
Barrett, A. J. (1981).Met. Enzymol. 80:581–588.
Bialasiewicz, A. A., Juppner, H., Diehl, V. and Hesch, R. D. (1979).Biochim. Biophys. A Acta 585:467–478.
Calvo, M. S., Fryer, M. J., Laakso, K. J., Nissenson, R. A., Price, P. A., Murray, T. M. and Heath, H. (1985).J. Clin. Invest. 76:2348–2354.
Christie, D. L., Hill, R. M., Isakow, K. and Barling, P. M. (1986).Anal. Biochem. 154:92–99.
Demay, M., Mitchell, J. and Goltzman, D. (1985).Am. J. Physiol. 249:E437-E446.
Di Bella, F. P., Arnaud, C. D. and Brewer, H. B. (1976).Endocrinology 99:429–436.
Heinrich, G., Kronenberg, H. M., Potts, J. T. and Habener, J. F. (1984).J. Biol. Chem. 259:3320–3329.
Hewick, R. M., Hunkapiller, M. W., Hood, L. E. and Dreyer, W. J. (1981).J. Biol. Chem. 256:7990–7997.
Martin, K. J., Freitag, J. J., Conrades, M. B., Hruska, K. A., Klahr, S. and Slatopolsky, E. (1978).J. Clin. Invest. 62:256–261.
Niall, H. D., Keutmann, H., Sauer, R., Hogan, M., Dawson, B., Aurbach, G. and Potts, J. T. (1970).Hoppe-Seyler's Z. Physiol. Chem. 351:1586–1588.
Parsons, J. A. and Robinson, C. J. (1968). In:Parathyroid Hormone and Thyrocalcitonin (Talmage, R. V. and Belanger, R. F. eds), Exerpta Medica, Amsterdam, pp. 329–331.
Partridge, N. C., Alcorn, D., Michelangeli, V. P., Ryan, G. and Martin, T. J. (1983).Cancer Res. 43:4308–4314.
Perry, H. M., Chappel, J. C., Bellorin-Font, E., Tamao, J., Martin, K. J. and Teitelbaum, S. L. (1984).J. Biol. Chem. 259:5531–5535.
Pillai, S. and Zull, J. E. (1986).J. Biol. Chem. 261:14919–14923.
Rao, L. G. and Murray, T. M. (1985).Endocrinology 117:1632–1638.
Rodan, G. A. and Rodan, S. B. (1984). In:Advances in Cyclic Nucleotide and Protein Phosphorylation Research (Greengard, P. et al. ed.), vol 17, Raven Press, New York, pp. 127–134.
Salomon, Y., Londos, C. and Rodbell, M. (1974).Anal. Biochem. 58:541–548.
Segre, G. V., Niall, H. D., Sauer, R. T. and Potts, J. T. (1977).Biochemistry 16: 2417–2427.
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Barling, P.M., Lowe, C. The biological properties of bovine parathyroid hormone (1–41), a fragment generated from the native hormone by human leukocytes. Biosci Rep 7, 43–50 (1987). https://doi.org/10.1007/BF01122726
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DOI: https://doi.org/10.1007/BF01122726