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Glutathione metabolism under the influence of hydroperoxides in the lactating mammary gland of the rat. Effect of glucose and extracellular ATP

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Abstract

Tert-butyl hydroperoxide decreases GSH and total free glutathione (GSH+2GSSG) contents of acini from lactating mammary glands. The decrease in total free glutathione can be explained by an increase in mixed disulfide formation and by excretion of GSS G to the extracellular medium, and subsequent degradation catalyzed by gamma-glutamyl transpeptidase. Low concentrations of glucose prevented the changes in glutathione levels induced by the peroxide. In the presence of extracellular ATP, glucose did not prevent these changes. However, incubations with the peroxide, did not alter the rate of other metabolic pathways by acini.

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Abbreviations

GSH:

Reduced glutathione

GSSG:

Glutathione disulfide

GSSR:

Glutathione mixed disulfide

GGT:

Gamma-glutamyl transpaptidase

tbOOH:

Tert-butyl hydroperoxide

References

  • Aebi, H. and Suter, H. (1974). In:Proceedings of the XVI Conference of the German Society of Biological Chemistry (L. Flohé, H. Ch. Benöhr, H. Seis H. D. Waller and A. Wendel, Eds.), Thieme, Stuttgart, pp. 192–201.

    Google Scholar 

  • Allen, L., Merck, R. and Tunis, A. (1980).Res. Commun. Chem. Path. Pharmacol. 27:175–182.

    Google Scholar 

  • Boyland, E. and Chasseaud, L. F. (1967).Biochem. J. 104:95–102.

    PubMed  Google Scholar 

  • Chance, B., Sies, H. and Boveris, A. (1979).Physiol. Rev. 59:527–605.

    PubMed  Google Scholar 

  • Chandramouli, V., Milligan, M. and Carter, J. R. Jr (1977).Biochemistry 16:1151–1158.

    PubMed  Google Scholar 

  • Chang, K. J. and Cuatrecasas, P. (1974).J. Biol. Chem. 2493170–3180.

    PubMed  Google Scholar 

  • Chang, K. J., Marcus, N. A., and Cuatrecasas, P. (1974).J. Biol. Chem. 249:6854–6865.

    PubMed  Google Scholar 

  • Chatwin, A. L., Linzell, J. L. and Setchell, B. P. (1969).J. Endocrinol. 44:247–254.

    PubMed  Google Scholar 

  • Chaudry, I. H. (1985).The Physiologist. 28:109–118.

    PubMed  Google Scholar 

  • Devoto, A., Puente, J. and Sapag-Hagar, M. (1986).Biochem. Soc. Trans. 14:658.

    Google Scholar 

  • Fridovich, I. (1978).Science 201:875–880.

    PubMed  Google Scholar 

  • Hahn, R., Wendel, A. and Flohé, L. (1978).Biochim. Biophys. Acta 539:324–337.

    PubMed  Google Scholar 

  • Halperin, M. L., Mak, M. L. and Taylor, W. M. (1978).Canad. J. Biochem. 50:708–712.

    Google Scholar 

  • Harrap, K. R., Jackson, R. C., Riches, P. G., Smith, C. A. and Hill, B. T. (1973).Biochim. Biophys. Acta 310:104–110.

    PubMed  Google Scholar 

  • Katz, J., Wals, P. A. and Van de Veede, R. L. (1974).J. Biol. Chem. 249: 7348–7357.

    PubMed  Google Scholar 

  • Kosower, N. S. and Kosower, E. M. K. (1978).Int Rev. Cytol. 54:109–160.

    PubMed  Google Scholar 

  • May, J. M. (1982).Arch. Biochem. Biophys. 214:56–66.

    PubMed  Google Scholar 

  • Modig, H. (1968).Biochem. Pharmacol. 17:177–186.

    PubMed  Google Scholar 

  • Mukherjee, S. P., Lane, R. H. and Lynn, W. S. (1978).Biochem. Pharmacol. 27:2589–2594.

    PubMed  Google Scholar 

  • Owens, C. W. I. and Belcher, R. V. (1965).Biochem. J. 94:705–711.

    PubMed  Google Scholar 

  • Pocius, P. A., Baumrucker, C. R., McNamara, J. P. and Bauman, D. E. (1980).Biochem. J. 188:565–568.

    PubMed  Google Scholar 

  • Racker, E. (1951).J. Biol. Chem. 190:685–696.

    PubMed  Google Scholar 

  • Reed, D. J. and Orrenius, S. (1977).Biochem. Biophys. Res. Commun. 77:1257–1264.

    PubMed  Google Scholar 

  • Robinson, A. M. and Williamson, D. H. (1977a).Biochem. J. 164:153–159.

    PubMed  Google Scholar 

  • Robinson, A. M. and Williamson, D. H. (1977b).Biochem. J. 168:465–474.

    PubMed  Google Scholar 

  • Rognstad, R. and Katz, J. (1969).Biochem. J. 111:431–444.

    PubMed  Google Scholar 

  • Sáez, G. T., Viña, J., Pallardó, F. V. and Romero, F. J. (1985).Biochem. Pharmacol. 34:453–454.

    Google Scholar 

  • Shapiro, R. A. and Curthoys, N. P. (1981).FEBS Lett. 133:131–134.

    PubMed  Google Scholar 

  • Sies, H., Gerstenecker, C., Menzel, H. and Flohé, L. (1972).FEBS Lett. 27: 171–175.

    PubMed  Google Scholar 

  • Sies, H., Wahlländer, A., Waydhas, C., Soboll, S. and Häberle, D. (1980).Adv. Enzyme Regulation 18:303–320.

    Google Scholar 

  • Sies, H., Brigelius, R. and Akerboom, T. P.M. (1983). In:Functions of Glutathione: Biochemical, Physiological, Toxicological, and Clinical Aspects (A. L. Larsson, S. Orrenius, A. Holmgren and B. Mannervik, Eds.), Raven Press, New York, pp. 51–54.

    Google Scholar 

  • Srivastava, S. K. and Beutler, E. (1969).Biochem. J. 114:833–837.

    PubMed  Google Scholar 

  • Tate, S. S. and Meister, A. (1974).Proc. Natl. Acad. Sci. USA 71:3329–3333.

    PubMed  Google Scholar 

  • Van Der Schans, G. P., Vos, O., Roos-Verheij, W. S. D. and Lohman, P. H. M. (1986).Int. J. Radiat. Biol. 50:453–465.

    Google Scholar 

  • Viña, J., Hems, R. and Krebs, H. A. (1978).Biochem. J. 170:627–630.

    PubMed  Google Scholar 

  • Viña, J., Puertes, I. R., Estrela, J. M., Viña, J. R. and Galbis, J. L. (1981a).Biochem. J. 194:99–102.

    PubMed  Google Scholar 

  • Viña, J., Puertes, I. R., Sáez, G. T. and Viña, J. R. (1981b).FEBS Lett. 126:250–252.

    PubMed  Google Scholar 

  • Viña, J., Puertes, I. R., Montoro, J. B. and Viña, J. R. (1983).FEBS Lett. 159:119–122.

    PubMed  Google Scholar 

  • Williamson, D. H., McKeown, S. R. and Ilic, V. (1975).Biochem. J. 150:145–152.

    PubMed  Google Scholar 

  • Williamson, D. H. (1980).FEBS Lett. 117:K93-K105.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Departamento de Fisiología, Facultad de Medicina, Universidad de Valencia, Avenida Blasco Ibañez 17, 46010, Valencia, Spain

    José M. Estrela & José Viña

  2. Departamento de Bioquímica-Biología Molecular, Facultad de Medicina, Universidad de Valencia, Avenida Blasco Ibañez 17, 46010, Valencia, Spain

    Juan B. Montoro & Juan R. Viña

Authors
  1. José M. Estrela
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  2. Juan B. Montoro
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  3. Juan R. Viña
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  4. José Viña
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Estrela, J.M., Montoro, J.B., Viña, J.R. et al. Glutathione metabolism under the influence of hydroperoxides in the lactating mammary gland of the rat. Effect of glucose and extracellular ATP. Biosci Rep 7, 23–31 (1987). https://doi.org/10.1007/BF01122724

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  • DOI: https://doi.org/10.1007/BF01122724

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