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The determination of alpha-adrenergic receptor concentration on rat pancreatic islet cells

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Bioscience Reports

Abstract

The selective a2 adrenergic antagonist yohimbine has been shown to prevent the noradrenaline induced inhibition of insulin secretion from isolated rat islets of Langerhans, Binding studies utilizing [3H]yohimbine showed specific binding to dispersed rat islet cells with a Kd of 2.9 nM and receptor concentration of 645 fmols/mg protein. The use of chloroquine to inhibit receptor recycling did not affect binding of the ligand. Binding studies and secretion data are consistent with the suggestion that adrenergic receptors of the α2 sub-type may play a dominant role in the regulation of insulin secretion.

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References

  • Cherksey, B., Mendelsohn, S., Zadunaisky, J. and Altszuler, N. (1983).Pharmacology 27:45–102.

    Google Scholar 

  • Dulis, B. H. and Wilson, I. B. (1980).J. Biol. Chem. 255:1043–1048.

    PubMed  Google Scholar 

  • Fyles, J. M., Cawthorne, M. A. and Howell., S. L. (1986).J. Endocrinol. 111:263–270.

    PubMed  Google Scholar 

  • Gey, G. O. and Gey, M. K. (1936).Am. J. Cancer 27:45–76.

    Google Scholar 

  • Hii, C. S. T. and Howell, S. L. (1984).Diabetes 33:291–296.

    PubMed  Google Scholar 

  • Howell, S. L. and Taylor, K. W. (1968).Biochem. J. 108:17–24.

    PubMed  Google Scholar 

  • Ishii, K., Yamamoto, S. and Kato, R. (1985).Naunyn-Schmiedebergs Arch Pharm. 328:253–257.

    Google Scholar 

  • Mendel, C. M. and Mendel, D. B. (1985)Naunyn-Schmiedebergs Arch. Pharm 328:253–257.

    Google Scholar 

  • Mendel, C. M. and Mendel, D. B. (1985).Biochem. J. 228:269–272.

    PubMed  Google Scholar 

  • Morgan, N. G. and Montague, W. (1985).Biochem. J. 226:571–576.

    PubMed  Google Scholar 

  • Nakaki, T., Nakadate, T. and Kato, R. (1980).Naunyn-Schmiedebergs Arch. Pharm 313:151–153

    Google Scholar 

  • Pipeleers, D. G., In't Veld, P. A., Van de Winkel, M., Maes, E., Schuit, F. C. and Gepts, W. (1985).Endocrinology 117:806–816.

    PubMed  Google Scholar 

  • Posner, B. I., Bergeron, J. S. M., Josefsberg, Z., Khan, M. N., Khan, R. J., Patel, B. A., Sikstrom, R. A. and Verma, A. K. (1981).Rec. Prog. Horm. Res. 37:539–582.

    PubMed  Google Scholar 

  • Scatchard, G. (1949).Ann. N. Y. Acad. Sci. 51:660–672.

    Google Scholar 

  • Schuit, F. C. and Pipeleers, D. G. (1986).Science 232:875–878.

    PubMed  Google Scholar 

  • Tsai, B. S. and Lefkowitz, R. J. (1978).Mol. Pharm. 14:540–548.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Physiology Department, King's College, Campden Hill Road, W8 7AH, London

    J. M. Fyles, M. A. Cawthorne & S. L. Howell

  2. Beecham Pharmaceuticals, Biosciences Research Centre, Great Burgh, KT18 5XQ, Epsom, Surrey, UK

    M. A. Cawthorne

Authors
  1. J. M. Fyles
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  2. M. A. Cawthorne
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  3. S. L. Howell
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Fyles, J.M., Cawthorne, M.A. & Howell, S.L. The determination of alpha-adrenergic receptor concentration on rat pancreatic islet cells. Biosci Rep 7, 17–22 (1987). https://doi.org/10.1007/BF01122723

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  • DOI: https://doi.org/10.1007/BF01122723

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