Determination of carotenoids in salmonoids

Bestimmung von Carotenoiden in Salmoniden

Zusammenfassung

Es wurde eine Routinemethode für die quantitative Analyse von Carotenoidpigmenten in Salmoniden entwickelt, die auf einer Extraktion der Farbstoffe mit Aceton und einem Reinigungsschritt auf Kieselgel vor der Identifizierung durch TLC beruht. Eine quantitative Bestimmung von Astaxanthin und Canthaxanthin konnte durch HPLC auf einer C-18 Säule und Elution mit Essigester/Methanol Wasser (9 + 1) (20:80) gefolgt von photometrischer Detektion im sichtbaren Bereich erzielt werden. Auch andere Kombinationen von Carotenoiden können mit diesem System getrennt werden, wenn isokratische oder Gradientenelution verwendet wird. Die Gehalte an Carotenoiden betrugen in den untersuchten Fischen 2,3–8,1 mg Astaxanthin/Kg und 0,3–1,0 mg Canthaxanthin/kg. Die gereinigten Farbstoffextrakte sind bei −18 °C mindestens 1 Monat lang lagerstabil.

Summary

A routine method for quantitative analysis of carotenoid pigments in salmonoids has been developed, based on an extraction with acetone and subsequent cleaning on silicagel prior to identification by thin-layer chromatography (TLC). Quantitative determinations of astaxanthin and canthaxanthin were performed by high-pressure liquid chromatography (HPLC) on a C-18 column eluted with 20% ethyl acetate and 80% methanol/water (9+1) followed by spectrophotometric detection in the visible region. Other combinations of carotenoids could be separated by this system using isocratic or gradient elution. Carotenoid contents of examined fish meat were 2.3–8.1 mg astaxanthin/kg and 0.3–1.0 mg canthaxanthin/kg. Purified colour extracts from fish could be stored at −18 °C for a least 1 month before a final carotenoid analysis was carried out.

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Correspondence to Pia Knuthsen.

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Christophersen, A.G., Knuthsen, P. & Skibsted, L.H. Determination of carotenoids in salmonoids. Z Lebensm Unters Forch 188, 413–418 (1989). https://doi.org/10.1007/BF01122538

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Keywords

  • Ethyl Acetate
  • Carotenoid
  • Astaxanthin
  • Carotenoid Content
  • Routine Method