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Sequences isolated from aDrosophila early-ecdysone puff are expressed in rat liver

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Bioscience Reports

Abstract

We have studied the presence of a cloned fragment of DNA fromDrosophila melanogaster in other organisms by means of nucleic acid hybridization analysis. The isolated region is localized in polytene chromosomes at the 63F subdivision. This region includes a puff that responds within minutes to ecdysone stimulation. We have found that 63F DNA fromD. melanogaster hybridizes 'in situ' to both DNA and RNA fromD. simulans, D. teissieri, andD. hydei. In all these species the isolated DNA remains associated with one early-ecdysone stimulated puff. The isolatedDrosophila recombinant DNA is also complementary to polyadenylated RNA from foetal and adult rat liver but fails to hybridize to the nonpolyadenylated RNA classes from both sources and to polyadenylated RNA from rat mammary glands.

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Authors and Affiliations

  1. Departmento de Bioquímica y Biología Molecular, Universidad Autónoma de Madrid, Facultad de Ciencias, Cantoblanco, Madrid 34, Spain

    Carmen Arribas & Marta Izquierdo

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  1. Carmen Arribas
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  2. Marta Izquierdo
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Arribas, C., Izquierdo, M. Sequences isolated from aDrosophila early-ecdysone puff are expressed in rat liver. Biosci Rep 4, 387–396 (1984). https://doi.org/10.1007/BF01122503

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  • DOI: https://doi.org/10.1007/BF01122503

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