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Aldrin epoxidase activity in liver microsomes from normal or streptozotocin-diabetic rats: Comparison with activity in isolated hepatocytes from normal rats incubated with glucagon

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Abstract

Aldrin epoxidase activity in liver microsomes from streptozotocin-diabetic rats is only 40% of that from normal rats. Epoxidation of aldrin has also been assayed in freshly isolated hepatocytes from normal rats. Addition of 10−7 M glucagon to the incubation medium leads to a decreased aldrin epoxidase activity. Owing to the previously reported phosphorylation of a purified cytochrome P-450 isozyme, it is postulated that the cytochrome P-450 dependent aldrin epoxidase may be regulated by a glucagon induced phosphorylation process.

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References

  1. Cohen, P. (1984). Hormones, second messengers and the reversible phosphorylation of proteins: an overview,Bio Essays. 2:63–68.

    Google Scholar 

  2. Pyerin, W., Taniguchi, H., Stier, A., Oesch, F. and Wolf, C. R. (1984). Phosphorylation of rabbit liver cytochrome P-450 LM2 and its effect on monooxygenase activity.Biochem. Biophys. Res. Commun. 122:620–626.

    PubMed  Google Scholar 

  3. Beale, E., Andreone, T., Koch, S., Granner, M. and Granner, D. (1984). Insulin and glucagon regulate cytosolic phosphoenol pyruvate carboxykinase (GTP) mRNA in rat liver.Diabetes 33:328–332.

    PubMed  Google Scholar 

  4. Reinke, L. A., Stohs, S. J. and Rosenberg, H. (1978). Altered activity of hepatic mixed-function monooxygenase enzymes in streptozotocin-induced diabetic rats.Xenobiotica 8:611–619.

    PubMed  Google Scholar 

  5. Past, M. R. and Cook, D. E. (1980). Alterations in hepatic microsomal cytochrome P-450 heme proteins in diabetic rats.Res. Commun. Chem. Pathol. Pharm. 27:329–337.

    Google Scholar 

  6. Rouer, E. and Leroux, J. P. (1980). Liver microsomal cytochrome P-450 and related monooxygenase activities in genetically hyperglycemic (ob/ob and db/db) and lean streptozotocin-treated mice.Biochem. Pharmacol. 29:1959–1962.

    PubMed  Google Scholar 

  7. Rouer, E., Beaune, P., Augereau, C. and Leroux, J. P. (1985). The effect of different hyperglucagonemic states on monooxygenase activities and isozymic pattern of cytochrome P-450 in mouse.Biosci. Rep. 5:335–341.

    PubMed  Google Scholar 

  8. Cresteil, T., Flinois, J. P., Pfister, A. and Leroux, J. P. (1979). Effect of microsomal preparations and induction on Cytochrome P-450 dependent monooxygenases in fetal and neonatal rat liver.Biochem. Pharmacol. 28:2057–2063.

    PubMed  Google Scholar 

  9. Demaugre, F., Leroux, J. P. and Cartier, P. (1978). The effects of pyruvate concentration, dichloroacetate and α-cyano-4 hydroxycinnamate on gluconeogenesis, ketogenesis and 3-hydroxybutyrate/3-oxobutyrate ratios in isolated hepatocytes.Biochem. J. 172:91–96.

    PubMed  Google Scholar 

  10. Wolff, T., Deml, E. and Wanders, H. (1979). Aldrin epoxidation, a highly sensitive indicator specific for cytochrome P-450 dependent monooxygenase activities.Drug. Metab. Dispos. 7:301–305.

    PubMed  Google Scholar 

  11. Omura, T. and Sato, J. (1964). The carbon monoxide binding pigment of liver microsomes. I. Evidence for its hemoprotein nature.J. Biol. Chem. 239:2370–2378.

    PubMed  Google Scholar 

  12. Greim, H. (1970). Synthesesteigerung und Abbauhemmung bei der Vermehrung der mikrosomalen Cytochrome P-450 und b-5 durch phenobarbital.Naunyn-Schmiedeberg's Arch. Pharmak. 266:261–275.

    Google Scholar 

  13. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951). Protein measurement with the folin phenol reagent.J. Biol. Chem. 193:265–275.

    PubMed  Google Scholar 

  14. Cartier, P., Leroux, J. P. and Temkine, H. (1967). Techniques de dosage des intermédiaires de la glycolyse dans les tissues.Ann. Biol. Clin. 25:791–813.

    Google Scholar 

  15. Chan, T. M. and Exton, H. (1976). A rapid method for the determination of glycogen content and radioactivity in small quantities of tissue or isolated hepatocytes.Anal. Biochem. 71:96–105.

    PubMed  Google Scholar 

  16. Kurihara, N., Hori, N. and Ichinose, R. (1984). Cytochrome P-450 content and aldrin epoxidation to dieldrin in isolated rat hepatocytes.Pestic. Biochem. Physiol. 21:63–73.

    Google Scholar 

  17. Ichinose, R. and Kurihara, N. (1985). Uptake of dieldrin, lindane and DDT by isolated rat hepatocytes.Pestic. Biochem. Physiol. 23:116–122.

    Google Scholar 

  18. Moldeus, P., Hogberg, J. and Orrenius, S. (1978). Isolation and use of liver cells. In:Methods in Enzymology (S. Fleischer and L. Packer, Eds.), Academic Press, New York52-60–65.

    Google Scholar 

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Author notes

  1. Evelyne Rouer

    Present address: INSERM U15, C. H. U. Cochin Port-Royal, 28 rue du Faubourg, St Jacques, 75674, Paris, Cedex 14, France

Authors and Affiliations

  1. INSERM U-75, CHU Necker Enfants-Malades, 156 rue de Vaugirard, 75730, Paris, Cedex 15, France

    Evelyne Rouer, Patricia Rouet & Jean Paul Leroux

Authors
  1. Evelyne Rouer
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  2. Patricia Rouet
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  3. Jean Paul Leroux
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Rouer, E., Rouet, P. & Leroux, J.P. Aldrin epoxidase activity in liver microsomes from normal or streptozotocin-diabetic rats: Comparison with activity in isolated hepatocytes from normal rats incubated with glucagon. Biosci Rep 7, 129–133 (1987). https://doi.org/10.1007/BF01121876

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  • DOI: https://doi.org/10.1007/BF01121876

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