Skip to main content
SpringerLink
Log in

Effects of parathyroid hormone on cyclic AMP-formation and cytoplasmic free Ca2+ in the osteosarcoma cell line UMR 106-01

  • Short Papers
  • Published:
Bioscience Reports

Abstract

The effects of parathyroid hormone (PTH) on cytoplasmic free CA2+ (Ca 2+i ) and cAMP-formation were investigated in the rat osteosarcoma cell line UMR 106-01.

In fura-2 loaded adherent single cells bPTH 1-34 (10 nM−1μM) induced a rapid transient increase in Ca 2+i in 11% of the studied cells. In fura-2 tracings from UMR 106-01 cells in suspension, bPTH 1-34 (0.1 μM) induced a transient increase in Ca 2+i in 20% of the experiments. The transient increase in Ca 2+i seen in suspensions of cells was not abolished by addition of EGTA (2.5 mM) prior to challenge with PTH, suggesting that the increase in Ca 2+i was derived from intracellular stores.

A marked rapid increase in cAMP-formation was observed in all experiments with cells in suspension, also in the experiments where PTH did not affect Ca 2+i .

These data show that PTH causes a release of Ca2+ from intracellular stores in a small percentage of osteosarcoma UMR 106-01 cells, and that PTH is capable of inducing an increase in cAMP-formation without affecting Ca 2+i in osteoblasts.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Vaes, G. (1988) Cellular biology and biochemical mechanism of bone resorption.Clin. Orthop. Rel. Res. 231:239–271.

    Google Scholar 

  2. Wong, G. L. (1986) Skeletal effects of parathyroid hormone. In: Bone and mineral research. Annual 4. Peck, W. A. (ed.) Elsevier, amsterdam. pp. 103–130.

    Google Scholar 

  3. Löwick, C. W. G. M., van Leeuwen, J. P. T. M., van der Meer, J. M., van Zeeland, J. K., Scheven, B. A. A. and Herrmann-Erlee, M. P. M. (1985) A two-receptor model for the action of parathyroid hormone on osteoblasts: a role for intracellular free calcium and cAMP.Cell Calcium 6:311–326.

    PubMed  Google Scholar 

  4. Yamaguchi, D. T., Hahn, T. J., Iida-Klein, A., Kleeman, C. R. and Muallem, S. (1987) Parathyroid hormone-activated calcium channels in an osteoblast-like clonal osteosarcoma cell line.J. Biol. Chem. 262:7711–7718.

    PubMed  Google Scholar 

  5. Reid, I. R., Civitelli, R., Halstead, L. R., Avioli, L. V. and Hruska, K. A. (1987) Parathyroid hormone acutely elevates intracellular calcium in osteoblast-like cells.Am. J. Physiol. 252:E45-E51.

    Google Scholar 

  6. Civitelli, R., Reid, I. R., Westbrook, S., Avioli, L. V. and Hruska, K. A. (1988) PTH elevates inositol polyphosphates and diacylglycerol in a rat osteoblast-like cell line.Am. J. Physiol. 255:E660-E667.

    PubMed  Google Scholar 

  7. Donahue, H. J., Fryer, M. J., Eriksen, E. F. and Heath, III H. (1988) Differential effects of parathyroid hormone and its analogues on cytosolic calcium ion and cAMP levels in cultured rat osteoblast-like cells.J. Biol. Chem. 263:13522–13527.

    PubMed  Google Scholar 

  8. Yamada, H., Tsutsumi, M., Fukase, M., Fujimori, A., Yamamoto, Y., Miyauchi, A., Fujii, Y., Noda, T., Fujii, N. and Fujita, T. (1989) Effects of human PTH-related peptide and human PTH on cyclic AMP production and cytosolic free calcium in an osteoblastic cell clone.Bone and Min. 6:45–54.

    Google Scholar 

  9. Leeuwen, J. P. T. M., Bos, M. P., Löwik, C. W. G. M. and Herrmann-Erlee, M. P. M. (1988) Effect of parathyroid hormone and parathyroid hormone fragments on the intracellular ionized calcium concentration in an osteoblast cell line.Bone and Min. 4:177–188.

    Google Scholar 

  10. Civitelli, R., Martin, T. J., Fausto, A., Gunsten, S. L., Hruska, K. A. and Avioli, L. V. (1989) Parathyroid hormone-related peptide transiently increases cytosolic calcium in osteoblast-like cells: comparison with parathyroid hormone.Endocrinology 125:1204–1210.

    PubMed  Google Scholar 

  11. Short, A. D., Brown, B. L. and Dobson, P. R. M. (1991) The effect of retinoic acid on parathyroid hormone- and parathyroid hormone-related peptide-induced intracellular calcium in a rat osteosarcoma cell line, UMR 106.Journal of Endocrinology 129:75–81.

    PubMed  Google Scholar 

  12. Bidwell, J. P., Fryer, M. J., Firek, A. F., Donahue, H. J. and Heath III, H. (1991) Desensitization of rat osteoblast-like cells (ROS 17/2.8) to parathyroid hormone uncouples the adenosine 3′5′-monophosphate and cytosolic ionized calcium response limbs.Endocrinology 128:1021–1028.

    PubMed  Google Scholar 

  13. Fujimori, A., Cheng, S., Avioli, L. V. and Civitelli, R. (1991) Dissociation of second messenger activation by parathyroid hormone fragments in osteosarcoma cells.Endocrinology 128:3032–3039.

    PubMed  Google Scholar 

  14. Boland, C. J., Fried, R. M. and Tashjian, A. H. (1986) Measurement of cytosolic free Ca2+ concentrations in human and rat osteosarcoma cells: actions of bone resorption-stimulating hormones.Endocrinology 118:980–989.

    PubMed  Google Scholar 

  15. Ljunggren, Ö, Johansson, H., Lindh, E., Lerner, U. H., Ljunghall, S. (1992) Bradykinin and thrombin, but not parathyroid hormone, cause a transient increase in cytoplasmic Ca2+ in the osteoblastic cell line MC3T3-E1.Calcif. Tissue Int. 50 (Suppl. 1), abstract no. 50.

    Google Scholar 

  16. Grynkiewicz, G., Poenie, M. and Tsien, R. Y. (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties.J. Biol. Chem. 260:3440–3450.

    PubMed  Google Scholar 

  17. Johanson, H., Larsson, R., Nygren, P., Lindh, E., Rastad, J., Åkerström, G. and Gylfe, E. (1987) Cytoplasmic Ca2+ concentration of single normal human and bovine parathyroid cells measured by dual wavelength microfluorometry.Biosci. Rep. 7:705–712.

    PubMed  Google Scholar 

  18. Hesketh, T. R., Smith, G. A., Moore, J. P., Taylor, M. V. and Metcalfe, J. C. (1983) Free cytoplasmic calcium concentration and the mitogenic stimulation of lymphocytes.J. Biol. Chem. 258:4876–4882.

    PubMed  Google Scholar 

  19. Chakraborty, M., Chatterjee, D., Kellokumpu, S., Rasmussen, H., Baron, R. (1991) Cell cycle-dependent coupling of the calcitonin receptor to different G proteins.Science 251:1078–1082.

    PubMed  Google Scholar 

  20. Lerner, U. H., Fredholm, B. B., Ransjö, M. (1986) Use of forskolin to study the relationship between cyclic AMP formation and bone resorption in vitro.Biochem. J. 240:529–539.

    PubMed  Google Scholar 

  21. Reeve, J., Meunier, P. J., Parson, J. A., Bernat, M., Bijvoet, O. L., Courpron, P., Edouard, C., Klenerman, L., Neer, R. M., Renier, J. C., Slovik, D., Vismans, F. J., Potts, J. T., Jr. (1980) Anabolic effect of human parathyroid hormone fragment on trabecular bone in involutional osteoporosis: a multicentre trial.Br. Med. J. 280:1340–1344.

    PubMed  Google Scholar 

  22. Gunness-Hey, M. and Hock, J. M. (1984) Increased trabecular bone mass in rats treated with human synthetic parathyroid hormone.Metab. Bone Dis. Relat. Res. 5:177–181.

    PubMed  Google Scholar 

  23. MacDonald, B. R., Gallagher, J. A. and Russel, R. G. G. (1986) Parathyroid hormone stimulates the proliferation of cells derived from human bone.Endocrinology 118:2445–2449.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Internal Medicine, University Hospital, S-751 85, Uppsala, Sweden

    Östen Ljunggren, Erik Lindh & Sverker Ljunghall

  2. Department of Surgery, University Hospital, S-751 85, Uppsala, Sweden

    Hans Johansson

  3. Department of Oral Cell Biology, University of Umeå, S-901 87, Umeå, Sweden

    Ulf H. Lerner

Authors
  1. Östen Ljunggren
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hans Johansson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ulf H. Lerner
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Erik Lindh
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sverker Ljunghall
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ljunggren, Ö., Johansson, H., Lerner, U.H. et al. Effects of parathyroid hormone on cyclic AMP-formation and cytoplasmic free Ca2+ in the osteosarcoma cell line UMR 106-01. Biosci Rep 12, 207–214 (1992). https://doi.org/10.1007/BF01121790

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01121790

Key Words

Search

Navigation