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6-Dimethyl amino purine and 2-amino purine inhibit the induction of expresion of milk protein genes by prolactin

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Abstract

Two protein kinase-inhibitors, 6-dimethyl amino purine and 2-amino purine inhibited induction of β-casein synthesis by prolactin when added to the culture medium of rabbit mammary explant and cells. The accumulation of the mRNA for αs1- and β-caseins and for whey acidic protein did not take place in the presence of the inhibitors whereas β-actin mRNA concentration was not altered. In the same experimental conditions, H7, an inhibitor of protein kinase C and, to a lower extent, of protein kinase A did not prevent prolactin from acting. These data suggest for the first time that specific protein kinases are involved in the transduction of the prolactin signal to milk protein genes.

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Abbreviations

6-DMAP:

6-dimethyl amino purine

2-AP:

2-amino purine

H7:

1-(5 isoquinoline sulfonyl)-2-methyl-piperazine dihydrochloride

WAP:

whey acidic protein

PRL:

prolactin

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Authors and Affiliations

  1. Unité de Différenciation Cellulaire, Institut National de la Recherche Agronomique, 78352, Jouy-en-Josas Cedex, France

    Mahasti Bayat-Sarmadi, Rachel Maliénou-Ngassa, Claudine Puissant & Louis-Marie Houdebine

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  1. Mahasti Bayat-Sarmadi
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  2. Rachel Maliénou-Ngassa
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  3. Claudine Puissant
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  4. Louis-Marie Houdebine
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Bayat-Sarmadi, M., Maliénou-Ngassa, R., Puissant, C. et al. 6-Dimethyl amino purine and 2-amino purine inhibit the induction of expresion of milk protein genes by prolactin. Biosci Rep 12, 189–197 (1992). https://doi.org/10.1007/BF01121788

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  • DOI: https://doi.org/10.1007/BF01121788

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