Abstract
Escherichia coli malate dehydrogenase has been isolated in homogeneous form by a procedure employing chromatography on DEAE-cellulose, 5'-AMP-Sepharose, and Sephacryl-200. It is composed of two identical polypeptide chains each of Mr = 32 500. Like porcine mitochondrial malate dehydrogenase, it is devoid of tryptophan, but otherwise it is not particularly more similar in composition to one of the eukaryotic isozymes than to the other. However, amino-terminal sequence analysis of the first 36 residues shows remarkable similarity of the bacterial and mitochondrial enzymes (69% identical residues) in contrast to the cytoplasmic form (27%). The two porcine heart enzymes are identical in 24t% of the positions compared. These results clearly establish that all three forms of malate dehydrogenase have evolved from a common precursor and that the prokaryotic and mitochondrial forms have retained sequences that are much closer to the ancestral one than the cytoplasmic enzyme. These findings appear to further substantiate the endosymbiotic hypothesis for the origin of the mitochondrion.
Similar content being viewed by others
References
Anderson S, Bankier AT, Barrell BG, de Bruijn MHL, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJH, Staden R & Young IG (1981) Nature290, 457–465.
Angeletti RH, Bradshaw RA & Wade RD (1971) Biochemistry10, 463–469.
Banaszak LJ & Bradshaw RA (1975)The Enzymes (PD Boyer, ed) Academic Press, New York, 11, 369–396.
Brauer AW, Margolius MN & Haber E (1975) Biochemistry14, 3029–3035.
Bridgen J, Harris JI & Northrop F (1975) FEBS Lett.49, 392–395.
Edelhoch H (1967) Biochemistry6, 1948–1954.
Glatthaar BE, Barbarash GR, Noyes BE, Banaszak LJ & Bradshaw RA (1974) Anal. Biochem.57, 432–451.
Laemmli UK (1970) Nature227, 680–685.
Margulis LS (1970)Origin of Eukaryotic Cells, Yale University Press, New Haven, Conn.
Moore S (1963) J. Biol. Chem.238, 235–237.
Murphey WH, Kitto GB, Everse J & Kaplan NO (1967a) Biochemistry6, 603–610.
Murphey WH, Barnaby C, Lin FJ & Kaplan NO (1967b) J. Biol. Chem.242, 1548–1559.
Phillips DO & Carr NG (1981) Annals N. Y. Acad. Sci.361, 298–311.
Raff RA & Mahler HR (1972) Science177, 575–582.
Schwartz RM & Dayhoff MO (1978) Science199, 395–403.
Schwarz Z & Kössel H (1980) Nature283, 739–742.
Steinman HM & Hill RL (1973) Proc. Natl. Acad. Sci. USA70, 3725–3729.
Thomas KA, Baglan NC & Bradshaw RA (1981) J. Biol. Chem. in press.
Uzzell T & Spolsky C (1974) Am. Sci.62, 334–343.
Uzzell T & Spolsky C (1981) Annals N.Y. Acad. Sci.361, 481–499.
Weininger MS & Banaszak LJ (1978) J. Mol. Biol.119, 443–449.
Wright IP & Sundaram TK (1979) Biochem. J.177, 441–448.
You KS & Kaplan NO (1975) J. Bacteriol.123 704–716.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fernley, R.T., Lentz, S.R. & Aradshaw, R.A. Malate dehydrogenase: Isolation fromE. coli and comparison with the eukaryotic mitochondrial and cytoplasmic forms. Biosci Rep 1, 497–507 (1981). https://doi.org/10.1007/BF01121583
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01121583