Abstract
Human thyroid cells in monolayer responded to acute stimulation by TSH with an increase in the secretion of T3. This process appeared to be dependent on a rise in the cytosolic calcium concentration since the antagonist of intraceliular calcium mobilization, TMB-8, was found to inhibit the release of T3 in response to TSH. The importance of intracellular calcium was further shown using the agent veratridine which increases the free calcium level within cells; veratridine potentiated the stimulation of T3 secretion by TSH and itself stimulated the release of T3 to a level higher than that seen in the presence of TSH alone. The calcium ionophore A23197 produced a biphasic effect on T3 secretion from human thyroid monolayers; at low concentrations, A23187 caused a decrease in both unstimulated and TSH-stimulated T3 secretion but above a concentration of 1 μM, T3 secretion was increased. The calmodulin antagonist W7 was found to inhibit T3 release in response to TSH, indicating a role for calmodulin in mediating the effects of intracellular calcium on T3 secretion.
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Babcock DF, First NC & Lardy HA (1976) J. Biol. Chem.251, 3881–3886.
Charo IF, Feinman D & Detwiler TC (1976) Biochem. Biophys. Res. Commun.72, 1462–1467.
Dumont JE (1971) Vitam. Horm.29, 287–412.
Feinman RD & Detwiler TC (1974) Nature249, 172–173.
Gorman RR, Wierenga W & Miller OV (1979) Biochem. Biophys. Acta572, 95–104.
Hidaka H, Yanaki T, Totsuka T & Asano M (1979) Molec. Pharmacol.15, 49–59.
Lowry OM, Rosebrough NJ, Farr AL & Randall RJ (1951) J. Biol. Chem.193, 265–275.
Maayan ML, Volpert EM & From A (1981) Endocrinology108, 1987–1988.
Mac Neil SM, Walker SW, Humphries H, Ollis CA, Brown BL, Tomlinson S & Munro DS (1983) Adv. Cyc. Nucl. Res. (In press).
Malagodi M & Chiou CY (1974) Eur. J. Pharmacol.27, 25–33.
Marcum JM, Dedman JR, Brinkley BR & Means AR (1978) Proc. Natl. Acad. Sci. (U.S.A.)75, 3771–3775.
Miot F, Dumont JE & Erneux F (1983) FEBS Lett.151, 273–276.
Ollis CA, Munro DS & Tomlinson S (1982) J. Endocrinol.95, 237–244.
Ollis CA, Mac Neil SM, Walker SW, Brown BL, Sharrard RM, Munro DS & Tomlinson S (1983) J. Endocrinol.99, 251–260.
Ollis CA, Fowles A, Brown BL, Munro DS & Tomlinson S (1984) J. Endocrinol. (submitted).
Pickett WC, Jesse RC & Cohen P (1977) Biochim. Biophys. Acta486, 209–213.
Richardson BP, Donatsch P, Hauser RE, Lowe DA, Marley PD & Matthews EK (1980) Horm. Metab. Res. Suppl.10, 81–91.
Rodesch F, Bogaert C & Dumont JE (1974) C.R. Acad. Sci.278, 931–934.
Rodesch F, Bogaert C & Dumont JE (1976) Mol. Cell. Endocrinol.5, 303–313.
Thomas JMF, Chap H & Douste-Blazy L (1981) Biochem. Biophys. Res. Commun.103, 819–824.
Tomlinson S, Mac Neil S, Walker SW, Ollis CA, Merritt JM & Brown BL (1984) Clin. Sci.66, 497–508.
Van Sande J, Decoster C & Dumont JE (1975) Biochem. Biophys. Res. Commun.62, 168.
Van Sande J, Decoster C & Dumont JE (1979) Mol. Cell. Endocrinol.14, 45–57.
Willems C, Rocmans P & Dumont JE (1971) FEBS Lett.14, 323–325.
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Ollis, C.A., Davies, R., Munro, D.S. et al. A role for intracellular calcium and calmodulin in the release of triiodothyronine from human thyroid-cell monolayer cultures. Biosci Rep 4, 695–702 (1984). https://doi.org/10.1007/BF01121023
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DOI: https://doi.org/10.1007/BF01121023