Abstract
Phosphatidylinositol (Ptd Ins) breakdown in response to thyrotropin-releasing hormone (TRH) was measured after preincubation of both normal rat anterior pituitary cells and GH3 turnout cells with [3H]inositol by the determination of [3H]inositol phosphate accumulation in the presence of lithium (which inhibits myo-inositol phosphatase). The method employed, which was originally developed for use with tissue slices, was adapted for isolated cells in monolayer culture. In GH3 cells, TRH stimulated the breakdown of phosphoinositide in a manner similar to that reported previously using alternative methods. Furthermore, in normal male anterior pituitary cells the dose-response profile for TRH stimulation of inositol phosphate accumuJation was found to correlate well with the dose-response profile for TRH stimulation of prolactin secretion. As this response was maintained in the absence of added calcium, the breakdown of phosphoinositide would appear to be implicated as an event preceding calcium mobilization.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Allison JH (1978) in: Cyclitols & Phosphoinositides (Eisenberg F & Wells WW, eds), pp 507–519, Academic Press, New York.
Allison JH & Blisner ME (1976) Biochim. Biophys. Res. Commun.68, 1332–1338.
Baird JG, Wojcikiewicz RJH, Dobson PRM & Brown BL Biochem. Soc. Transac. (in press).
Berridge MJ (1981) Mol. Cell. Endocrinol.24, 115–140.
Berridge MJ, Downes CP & Hanley MR (1982) Biochem. J.206, 587–595.
Drummond AH & MacPhee CH (1981) Br. J. Pharmacol.74, 967P-968P.
Gershengorn MC (1980) J. Biol. Chem.255, 1801–1803.
Gourdji D, Tougard C & Tixier-Vidal A (1982) in: Frontiers in Neuroendocrinology, vol.7, 317–357 (Ganong WF & Martini L, eds), Raven Press, New York.
Hawthorne JN & Pickard MR (1978) J. Neurochem.32, 5–14.
Hokin MR, Hokin LE, Saffron M, Schally AU & Zimmerman BM (1958) J. Biol. Chem.233, 811–813.
Jones LM, Cockcroft S & Michell RH (1979) Biochem. J.182, 669–679.
Labrie F, Borgeat P, Drouin J, Lagace L, Giguere V, Raymond V, Godbout M, Massicotte J, Ferland L, Barden N, Beaulieu M, Cote J, Lepine J, Meunier H & Veilleux R (1982) in: Cyclic Nucleotides, Part II, Physiology & Pharmacology (Kebabian JW & Nathanson JA, eds), Chap. 27, Springer-Verlag, Berlin, Heidelberg, New York.
Leung PCK, Raymond U & Labrie F (1982) Life Sci.31, 3037–3041.
Michell RH (1975) Biochim. Biophys. Acta415, 81–147.
Michell RH (1979) Trends Biochem. Sci.4, 128–131.
Michell RH & Kirk CJ (1981a) Trends Pharmacol. Sci.2, 86–89.
Michell RH & Kirk CJ (1981b) Biochem. J.198, 247–248.
Michell RH, Kirk CJ, Jones LM, Downes CP & Creba JA (1981) Phil. Trans. Roy. Soc. LondonB296, 123–137.
Naor Z, Leifer AM & Catt KJ (1980) Endocrinology107, 1438–1445.
Ozawa & Kimura N (1979) Proc. Natl. Acad. Sci. U.S.A.76, 6017–6020.
Putney JW (1981) Life Sci.29, 1183–1194.
Rebecchi MJ, Kolesnick RN & Gershengorn MC (1983) J. Biol. Chem.258, 227–234.
Schrey MP, Brown BL & Ekins RP (1977) Mol. Cell. Endocrinol.8, 271–282.
Schrey MP, Brown BL & Ekins RP (1978) Mol. Cell. Endocrinol.11, 249–264.
Sherman WR, Leavitt AL, Honchar MP, Hallcher LM & Phillips BE (1981) J. Neurochem.36, 1947–1951.
Snyder GD & Bleasdale JE (1982) Mol. Cell. Endocrinol.28, 55–63.
Sutton CA & Martin TFJ (1982) Endocrinology110, 1273–1280.
Thorner MO, Hackett JT, Murad F & MacLeod RM (1980) Neuroendocrinology31, 390–402.
Young PW, Bicknell RJ & Scholfield JG (1979) J. Endocrinol.80, 203–213.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Baird, J.G., Dobson, P.R.M., Wojcikiewicz, R.J.H. et al. Thyrotropin-releasing hormone stimulates inositol phosphate production in normal anterior pituitary cells and GH3 tumour cells in the presence of lithium. Biosci Rep 3, 1091–1099 (1983). https://doi.org/10.1007/BF01120201
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01120201