Skip to main content
SpringerLink
Log in

The primary structure of the fourth component of human complement (C4)-C-terminal peptides

  • Published:
Bioscience Reports

Abstract

C-terminal CNBr peptides of the three polypeptide chains of C4 were obtained and sequenced. These results supplement previously obtained data, notably t h e protein sequence derived from cDNA sequencing of pro-C4 (Belt KT, Carroll MC & Porter RR (1984) Cell36, 907–914) and the N-terminal sequences of the three polypeptides (Gigli I, von Zabern I & Porter RR (1977) Biochem. J.165, 439–446), to define the complete primary structure of the plasma form of C4. The β (656 residues), α (748 residues), and γ (291 residues) chains are found in positions 1–656, 661–1408, and 1435–1725 in the pro-C4 molecule.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anson DS, Choo KH, Rees DJG, Giannelli F, Gould K, Huddleston JA & Brownlee GG (1984) EMBO J.3, 1053–1060.

    PubMed  Google Scholar 

  • Arlaud GJ & Gagnon J (1983) Biochemistry22, 1758–1764.

    PubMed  Google Scholar 

  • Arlaud GJ & Gagnon J (1985) FEBS Lett.180, 234–238.

    PubMed  Google Scholar 

  • Beckmann RJ, Schmidt RJ, Santerre RF, Plutzky J, Crabtree GR & Long GL (1985) Nucleic Acid Res.13, 5233–5247.

    PubMed  Google Scholar 

  • Belt KT, Carroll MC & Porter RR (1984) Cell36, 907–914.

    PubMed  Google Scholar 

  • Belt KT, Yu CY, Carroll MC & Porter RR (1985) Immunogenetics21, 173–180.

    PubMed  Google Scholar 

  • Bolotin C, Morris S, Tack B & Prahl J (1977) Biochemistry16, 2008–2015.

    PubMed  Google Scholar 

  • Campbell RD, Dodds AW & Porter RR (1980) Biochem. J.189, 67–80.

    PubMed  Google Scholar 

  • Campbell RD, Gagnon J & Porter RR (1981) Biochem. J.199, 359–370.

    PubMed  Google Scholar 

  • Carroll MC, Campbell RD, Bentley DR & Porter RR (1984) Nature307, 237–241.

    PubMed  Google Scholar 

  • Chan AC & Atkinson JP (1985) Fed. Proc. (Abs.)44, 1531.

    Google Scholar 

  • Chan AC, Mitchell KR, Munns TW, Karp DR & Atkinson JP (1983) Proc. Natl. Acad. Sci. U.S.A.80, 268–272.

    PubMed  Google Scholar 

  • Christie DL & Gagnon J (1982) Biochem. J.201, 555–567.

    PubMed  Google Scholar 

  • de Bruijn MHL & Fey GH (1985) Proc. Natl. Acad. Sci. U.S.A.82, 708–712.

    PubMed  Google Scholar 

  • Degen SJF, MacGillivray RTA & Davis EW (1983) Biochemistry22, 2087–2097.

    PubMed  Google Scholar 

  • Docherty K & Steiner DF (1982) Ann. Rev. Physiol.44, 625–638.

    Google Scholar 

  • Gigli I, von Zabern I & Porter RR (1977) Biochem. J.165, 439–446.

    PubMed  Google Scholar 

  • Hall RE & Colten HR (1977) Proc. Natl. Acad. Sci. U.S.A.74, 1707–1710.

    PubMed  Google Scholar 

  • Janatova J & Tack BF (1981) Biochemistry20, 2394–2402.

    PubMed  Google Scholar 

  • Laemmli UK (1970) Nature277, 680–685.

    Google Scholar 

  • Law SK, Lichtenberg NA & Levine RP (1980) Proc. Natl. Acad. Sci. U.S.A.77, 7194–7198.

    PubMed  Google Scholar 

  • Long GL, Belagaje RM & MacGillivray RTA (1984) Proc. Natl. Acad. Sci. U.S.A.81, 5653–5656.

    PubMed  Google Scholar 

  • Lundwall A, Wetsel RA, Domdey H, Tack BF & Fey GH (1984) J. Biol. Chem.259, 13851–13856.

    PubMed  Google Scholar 

  • Mauff G, Alper CA, Awdeh A, Batchelor JR, Bertrams J, Braun-Petersen G, Dawkins RL, Demant P, Edwards J, Gross-Wilde H, Hauptmann G, Klouda P, Lamm L, Mollenhauer E, Nerl C, Olaisen B, O'Neill G, Rittner C, Roos MH, Skanes V, Teisberg P & Wells L (1983) Immunobiology164, 184–191.

    PubMed  Google Scholar 

  • Nonaka M, Takahashi M, Natsuume-Dakai S, Nonaka M, Tanaka S, Shimizu A & Honjo T (1984) Proc. Natl. Acad. Sci. U.S.A.81, 6822–6826.

    PubMed  Google Scholar 

  • O'Neill GJ, Yang SY and Dupont B (1978) Proc. Natl. Acad. Sci. U.S.A.75, 5165–5169.

    PubMed  Google Scholar 

  • Schendel DJ, O'Neill GJ & Wank R (1984) Immunogenetics20, 23–31.

    PubMed  Google Scholar 

  • Schreiber RD & Muller-Eberhard HJ (1974) J. Exp. Med.140, 1324–1335.

    PubMed  Google Scholar 

  • Scott AP, Ratcliffe JG, Rees LH, Landon J, Bennett HPJ, Lowry PJ & McMartin C (1973) Nature New Biology244, 65–67.

    PubMed  Google Scholar 

  • Sepich DS, Noonan DJ & Ogata RT (1985) Proc. Natl. Acad. Sci. U.S.A. (in press).

  • Sim RB & Sim E (1981) Biochem. J.193, 129–141.

    PubMed  Google Scholar 

Download references

Author information

Author notes

  1. Jean Gagnon

    Present address: Biotechnology Research Institute, National Research Council Canada, 750 rue Bel-Air, H4C 2K3, Montreal, Quebec, Canada

Authors and Affiliations

  1. M.R.C. Immunochemistry Unit, Department of Biochemistry, University of Oxford, South Parks Road, OX1 3QU, Oxford, UK

    S. K. Alex Law & Jean Gagnon

Authors
  1. S. K. Alex Law
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jean Gagnon
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Law, S.K.A., Gagnon, J. The primary structure of the fourth component of human complement (C4)-C-terminal peptides. Biosci Rep 5, 913–921 (1985). https://doi.org/10.1007/BF01119904

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01119904

Keywords

Search

Navigation