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Electrophoretic resolution of microheterogeneity inVibrio cholerae lipopolysaccharide

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Bioscience Reports

Abstract

Lipopolysaccharide (LPS) fromVibrio cholerae has been analysed by sodium dodecyl sulfate-potyacrylamide gel electrophoresis. Under normal conditions of electrophoresis which resolveEscherichia coli LPS, V.cholerae LPS shows two diffuse and unresolved bands. However, on long gels at low concentration it can be resolved into two major band types. There are at least 10 slow moving, discrete bands of regular periodicity and three fast moving bands. Comparison with LPS fromE. coli indicates that the heterogeneity occurs over a much smaller range of molecular weight in V.cholerae LPS, with the entire spectrum of discrete bands being contained within the space of fourE. coli repeating units.

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Authors and Affiliations

  1. Department of Biochemistry, University of Glasgow, G12 8QQ, Glasgow, UK

    Souravi Ghosh & Ailsa M. Campbell

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  1. Souravi Ghosh
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  2. Ailsa M. Campbell
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Ghosh, S., Campbell, A.M. Electrophoretic resolution of microheterogeneity inVibrio cholerae lipopolysaccharide. Biosci Rep 5, 761–764 (1985). https://doi.org/10.1007/BF01119874

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  • DOI: https://doi.org/10.1007/BF01119874

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