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Effects of polymyxin B on the sarcoplasmic reticulum membrane

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Bioscience Reports

Abstract

Polymyxin B, a cyclic peptide antibiotic, inhibits Ca2+-ATPase, p-nitrophenyl phosphatase and phosphorylase kinase activities associated with rabbit skeletal muscle sarcoplasmic reticulum membranes; 50% inhibition is induced by 100 μM, 130μM and 550 μM of polymyxin respectively. The fluorescence intensity of fluorescein isothiocyanate-labeled Ca2+-ATPase, decreases in the presence of polymyxin (50% of the total decrease at 70 μM polymyxin). On the other hand, the polypeptide inhibits calmodulin-dependent endogenous phosphorylation of 60 kDa, 20 kDa and 14 kDa membrane proteins, while an increase of calmodulin-dependent phosphorylation is observed in 132 kDa and 86 kDa proteins.

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Authors and Affiliations

  1. Institute of Biological Research, The National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, 116 35, Athens, Greece

    T. B. Ktenas, T. G. Sotiroudis & A. E. Evangelopoulos

Authors
  1. T. B. Ktenas
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  2. T. G. Sotiroudis
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  3. A. E. Evangelopoulos
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Ktenas, T.B., Sotiroudis, T.G. & Evangelopoulos, A.E. Effects of polymyxin B on the sarcoplasmic reticulum membrane. Biosci Rep 9, 573–578 (1989). https://doi.org/10.1007/BF01119800

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  • DOI: https://doi.org/10.1007/BF01119800

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