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Synthesis of (+) and (−) RNA molecules of potato spindle tuber viroid (PSTV) in isolated nuclei and its impairment by transcription inhibitors

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Bioscience Reports

Abstract

Transcription studies with highly purified potato cell nuclei in combination with a ‘transcription-hybridization analysis’ unequivocally demonstrate that the nucleus is the subcellular site where the entire process of PSTV replication takes place. Inhibition experiments with actinomycin D and α-amanitin furthermore suggest that the nuclear DNA-dependent RNA polymerases I and II are involved in the synthesis of PSTV (+) and (−) RNA, respectively.

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Authors and Affiliations

  1. Abteilung Viroidforschung, Max-Planck-Institut für Biochemie, D-8033, Planegg-Martinsried, F.R.G.

    Ellen Spiesmacher, Hans-Peter Mühlbach, Martin Tabler & Heinz L. Sänger

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  1. Ellen Spiesmacher
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  2. Hans-Peter Mühlbach
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  3. Martin Tabler
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  4. Heinz L. Sänger
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Spiesmacher, E., Mühlbach, HP., Tabler, M. et al. Synthesis of (+) and (−) RNA molecules of potato spindle tuber viroid (PSTV) in isolated nuclei and its impairment by transcription inhibitors. Biosci Rep 5, 251–265 (1985). https://doi.org/10.1007/BF01119595

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  • DOI: https://doi.org/10.1007/BF01119595

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