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The effect of tunicamycin on transferrin synthesis and secretion in hormonally stimulated explants of rabbit mammary gland

  • Published:
Bioscience Reports

Abstract

  1. 1.

    Pregnant rabbit mammary gland explants cultured with insulin, prolactin and cortisol synthesize and secrete transferrin radiolabelled with [3H]leucine or [ 3H]mannose.

  2. 2.

    Addition of tunicamycin inhibits the glycosylation but not synthesis of the [3H]leucine-labelled poly-peptide.

  3. 3.

    Secretion of the non-glycosylated protein is only marginally inhibited.

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Authors and Affiliations

  1. Department of Biochemistry, University of Nottingham Medical School, Queen's Medical Centre, NG7 2UH, Nottingham, U.K.

    Judith P. Bradshaw & David A. White

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  1. Judith P. Bradshaw
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  2. David A. White
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Bradshaw, J.P., White, D.A. The effect of tunicamycin on transferrin synthesis and secretion in hormonally stimulated explants of rabbit mammary gland. Biosci Rep 5, 229–236 (1985). https://doi.org/10.1007/BF01119592

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  • DOI: https://doi.org/10.1007/BF01119592

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