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ATP formation from deoxyadenosine in human erythrocytes: Evidence for a hitherto unidentified route involving adenine and S-adenosylhomocysteine hydrolase

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Bioscience Reports

Abstract

A novel route of ATP formation has been identified using erythrocytes from patients deficient in four different enzymes associated with ATP formation. It entails prior adenine production from deoxyadenosine (or adenosine) in a reaction involving S-adenosylhomocysteine hydrolase. The postulated route has been demonstrated in human erythrocytes which, unlike other human cells, cannot form ATP from IMP. It is based on studies by others using purified S-adenosylhomocysteine hydrolase preparationsin vitro. The results provide the first confirmation that this reaction occurs in intact human cellsin vitro and thus most probablyin vivo. This adenine production is normally masked in intact cells by further metabolism to ATP. Clinical significance for such a route is suggested by the fact that some adenosine analogues with potent oncostatic and antiviral properties also release adenine (or analogues)in vitro.

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Authors and Affiliations

  1. Purine Research Laboratory, Clinical Science Laboratories, UMDS Guy's Hospital, London Bridge, SE1 9RT, London, UK

    H. A. Simmonds, L. D. Fairbanks, J. A. Duley & G. S. Morris

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  1. H. A. Simmonds
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  2. L. D. Fairbanks
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  3. J. A. Duley
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  4. G. S. Morris
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Simmonds, H.A., Fairbanks, L.D., Duley, J.A. et al. ATP formation from deoxyadenosine in human erythrocytes: Evidence for a hitherto unidentified route involving adenine and S-adenosylhomocysteine hydrolase. Biosci Rep 9, 75–85 (1989). https://doi.org/10.1007/BF01117513

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  • DOI: https://doi.org/10.1007/BF01117513

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