Abstract
Plasmid stability was studied in antibiotic-free chemo-stat cultures . Disruption, either by deletion or insertion, of the tetracycline resistance gene in the EcoRl/EcoRV region of the cloning vector pBR322 or in the HindIII]BamHl region of pACYCI84 yields plasmids markedly more stable than the parent plasmids. Thus, at least for these two instances, cloning of a partitioning (par) locus is not prerequisite for plasmid maintenance.
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Issued as NRCC publication No. 23992.
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Vernet, T., McDonald, I.J., Cameron, D.R. et al. Stable maintenance in chemostat-grownEscherichia coli of pBR322 and pACYC184 by disruption of the tetracycline resistance gene. Biosci Rep 5, 29–37 (1985). https://doi.org/10.1007/BF01117438
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DOI: https://doi.org/10.1007/BF01117438