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A characterization of heam uptake and intracellular distribution by isolated hepatocytes

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Bioscience Reports

Abstract

The uptake and intracellular distribution of haem by isolated rat hepatocyte suspensions was studied. An increase in cell haem content occurred after a challenge with 5, 10 or 20 μM haem, supplied as methaemalbumin. The rate of haem uptake was temperature dependent; no non-specific binding occurred. Intracellular haem distribution data are consistent with a rapid association of haem with the endoplasmic reticulum fraction prior to its accumulation in the cytosol and at the mitochondrion.

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  1. Biological Laboratory, University of Kent, CT2 7NJ, Canterbury, Kent, UK

    A. Lodola

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  1. A. Lodola
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Lodola, A. A characterization of heam uptake and intracellular distribution by isolated hepatocytes. Biosci Rep 5, 609–614 (1985). https://doi.org/10.1007/BF01117074

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  • DOI: https://doi.org/10.1007/BF01117074

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