Skip to main content
SpringerLink
Log in

Transglutaminase and cellular motile events: Retardation of proinsulin conversion by glycine methylester

  • Published:
Bioscience Reports

Abstract

Glycine methylester, an inhibitor of transglutaminase, decreased glucose-stimulated insulin release and delayed proinsulin conversion in rat pancreatic islets pulselabelle with L-[4-[3H]phenylalanine. Sarcosine methylester, which does not inhibit transglutaminase activity, failed to affect insulin release and proinsulin conversion. The incorporation of L-[4-3H]phenylalanine into islet peptides, the ratio of hormonal to total tritiated peptides and the insulin content of the islets failed to be affected by either of these methylesters. It is proposed that transglutaminase participates in the control of motile events involved in both the transfer of proinsulin from its site of synthesis to its site of conversion, and the translocation of insulin from its site of storage to its site of release.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bersten AM, Ahkong QF, Hallinan T, Nelson SJ & Lucky JA (1983) Inhibition of the formation of myotubes in vitro by inhibitors of transglutaminase. Biochim. Biophys. Acta762, 429–436.

    Google Scholar 

  2. Bungay PJ, Potter JM & Griffin M (1984) The inhibition of glucose-stimulated insulin secretion by primary amines. A role for transglutaminase in the secretory mechanism. Biochem. J.219, 819–827.

    Google Scholar 

  3. Gomis R, Deleers M, Malaisse-Lagae F, Sener A, Garica-Morales P, Roviro A, Valverde I & Malaisse WJ (1984) Metabolic and secretory effects of methylamine in pancreatic islets. Cell Biochemistry and Function2, 161–166.

    Google Scholar 

  4. Gomis R, Sener A, Malaisse-Lagae F & Malaisse WJ (1983) Transglutaminase activity in pancreatic islets. Biochim. Biophys. Acta760, 384–388.

    Google Scholar 

  5. Lacy PE & Kostianovsky M (1967) Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes16, 35–39.

    Google Scholar 

  6. Lorand L & Conrad SM (1984) Transglutaminases. Mol. Cell. Biochem.58, 9–35.

    Google Scholar 

  7. Malaisse WJ, Malaisse-Lagae F, Mayhew DA & Wright PH (1967) Effects of sulfonylureas upon insulin secretion by the rat's pancreas. In: Tolbutamide after Ten Years (Butterfield WJH & Van Westering W, eds), Excerpta Medica Foundation I.C.S., vol 149, pp. 49–60.

  8. Malaisse WJ, Malaisse-Lagae F, Van Obberghen E, Somers G, Devis G, Ravazzola M, & Orci L (1975) Role of microtubules in the phasic pattern of insulin release. Ann. N.Y. Acad. Sci.253, 630–652.

    Google Scholar 

  9. Malaisse WJ, Malaisse-Lagae F, Walker MO & Lacy PE (1971) The stimulus-secretion coupling of glucose-induced insulin release. V. The participation of a microtubular-microfilamentous system. Diabetes20, 257–265.

    Google Scholar 

  10. Malaisse WJ & Orci L (1979) The role of the cytoskeleton in pancreatic B-cell function. Meth. Achiev. Exp. Pathol.9, 112–136.

    Google Scholar 

  11. Malaisse-Lagae F., Amherdt M, Ravazzola M, Sener A Hutton JC, Orci L & Malaisse WJ (1979) Role of microtubules in the synthesis, conversion and release of (pro)insulin. A biochemical and radioautographic study in rat islets. J. Clin. Invest.63, 1284–1296.

    Google Scholar 

  12. Malaisse-Lagae F & Malaisse WJ (1984) Insulin release by pancreatic islets. In: Methods in Diabetes Research (Larner J & Pohl S, eds), vol 1, pp. 147–152, Wiley, New York.

    Google Scholar 

  13. Orci L (1974) A portrait of the pancreatic B-cell. Diabetologia10, 163–167.

    Google Scholar 

  14. Pipeleers DG, Marichal M & Malaisse WJ (1973) The stimulus-secretion coupling of glucose-induced insulin release. XIV. Glucose regulation of insular biosynthetic activity. Endocrinology33, 1001–1011.

    Google Scholar 

  15. Sener A, Dunlop ME, Gomis R., Mathias PCF, Malaisse-Lagae F & Malaisse WJ (1985) Role of transglutaminase in insulin release. Study with glycine and sarcosine methylesters. Endocrinology117, in press.

  16. Sener A, Gomis R., Lebrun P, Herchuelz A, Malaisse-Lagae F & Malaisse WJ (1984) Methylamine and islet function: possible relationship to Ca2+-sensitive transglutaminase. Mol. Cell. Endocrinol.36, 175–180.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Fundacion Jimenez Diaz, Universidad Autonoma de Madrid, Madrid, Spain

    C. Alarcon, I. Valverde & W. J. Malaisse

  2. Laboratory of Experimental Medicine, Brussels Free University, Brussels, Belgium

    C. Alarcon, I. Valverde & W. J. Malaisse

Authors
  1. C. Alarcon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. Valverde
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. J. Malaisse
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Alarcon, C., Valverde, I. & Malaisse, W.J. Transglutaminase and cellular motile events: Retardation of proinsulin conversion by glycine methylester. Biosci Rep 5, 581–587 (1985). https://doi.org/10.1007/BF01117071

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01117071

Keywords

Search

Navigation