References
Köhler G & Milstein C (1975) Continuous cultures of fused cells secreting antibodies of predefined specificity. Nature256, 995.
Köhler G & Milstein C (1976) Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur. J. Immun.6, 511.
Polin RA (1980). Monoclonal antibodies against streptococcal antigens. In: Monoclonal Antibodies (Kenneth, McKearn & Bechtol, eds), pp 353–359, Plenum Press, New York.
Flamand A, Wiktor TJ & Koprowski H (1980) Use of hybridoma monoclonal antibodies in the detection of antigenic differences between rabies and rabies-related virus proteins. J. Gen. Virol.48, 97.
Mitchell G, Cruise KM, Garcia EG & Anders RF (1981) Hybridoma derived antibody with immunodiagnostic potential for schistosomiasis japonica. Proc. Nat. Acad. Sci. USA78, 3165.
Howard FD, Ledbetter JA, Wong J, Bieber C, Stinson EB & Herzenberg LA (1981) Human T lymphocyte differentiation marker defined by monoclonal antibodies that block E-rosette formation. J. Immunol.126, 2117.
Brodsky FM, Parham P, Barnstable CJ, Crumpton MJ & Bodmer WF (1979) Monoclonal antibodies for analysis of the HLA system. Immunol. Reviews47, 3.
Koprowski H, Steplewski Z, Herlyn D & Herlyn M (1978) Production of monoclonal antibody against human melanoma by somatic cell hybrids. Proc. Nat. Acad. Sci. USA75, 3405.
Secher DS & Burke DC (1980) A monoclonal antibody for large-scale purification of human leucocyte interferon. Nature285, 446.
McMaster WR & Williams AF (1979) Monoclonal antibodies to Ia antigens from rat thymus: Crossreactions with mouse and human and use in purification of rat Ia glycoproteins. Immunol. Reviews47, 117.
Miggiano V, Stähli C, Häring P, Schmidt J, Le Dain M, Glatthaar B & Staehlin T (1980). Monoclonal antibodies to three tumor markers: human chorionic gonadotropin, prostatic acid phosphatase and carcinoembryonic antigen. In: Protides of the Biological Fluids (Peters, ed), pp 501–504, Pergamon Press, Oxford.
Leptin M, Potash MJ, Grützmann R, Heusser C, Shulman M, Köhler G & Melchers F (1984) Monoclonal antibodies specific for murine IgM. I. Characterization antigenic determinants on the four constant domains of the μ chain. Eur. J. Immun.14, 554.
Yoshida N, Nussenzweig RS, Potocnjak P, Nussenzweig V & Aikawa M (1980) Hybridoma produces protective antibodies directed against the sporozoite stage of malaria parasite. Science207, 71.
Mudgett-Hunter M, Margolies MN, Smith TW, Nowotny J & Haber E (1980) Monoclonal antibodies to the cardiac glycoside digoxin. In: Monoclonal Antibodies and T Cell Hybridomas (Hämmerling G & Kearney J, eds), pp 367–374, Elsevier, Amsterdam.
Hollander N, Pillemer E & Weissman IL (1980) Blocking effect of Lyt-2 antibodies on T-cell functions. J. Exp. Med.152, 674.
Koskimies S (1980) Human lymphoblastoid cell line producing specific antibody against Rh-antigen D. Scand. J. Immunol.11, 73.
Reth M, Kelsoe G & Rajewsky K (1981) Idiotypic regulation by isologous monoclonal anti-idiotypic antibodies. Nature290, 257.
Holtkamp B, Cramer M, Lemke H & Rajewsky K (1981) Isolation of a cloned cell line expressing variant H2k using the fluorescence activated cell sorter. Nature289, 66.
Gerhard W & Webster RG (1978) Antigenic drift in influenza A virus. I. Selection and characterization of antigenic variants of A/PR/8/34 (HON1) influenza virus with monoclonal antibodies. J. Exp Med.148, 383.
Zipser B & McKay R (1981) Monoclonal antibodies distinguish identifiable neurones in the leech. Nature289, 549.
Mach JP, Buchegger F, Forni M, Ritchard J, Berche C, Lumbroso J-D, Schrejer M, Giradet Ch, Accolla RS & Carrel S (1981) Use of radiolabelled monoclonal anti-CEA antibodies for the detection of human carcinomas by external photoscanning and tomoscintography. Immunology Today2, 239.
Coffino P & Scharff MD (1971) Rate of somatic mutation in immunoglobulin production by mouse myeloma cells. Proc. Nat. Acad. Sci. USA68, 219.
Morrison SL (1978) Murine heavy chain disease. Eur. J. Immun.8, 194.
Morrison SL (1979) Sequentially derived mutants of the constant region of the heavy chain of murine immunoglobulins. J. Immun.123, 793.
Mosmann TR & Williamson AR (1980) Structural mutations in a mouse immunoglobulin light chain resulting in failure to be secreted. Cell20, 283.
Wu GE, Hozumi N & Murialdo H (1983) Secretion of a λz immunoglobulin chain is prevented by a single amino acid substitution in its variable region. Cell33, 77.
Milstein C, Adetugbo K, Cowan NJ, Köhler G, Secher DS & Wilde CD (1977) Somatic cell genetics of antibody-secreting cells: Studies of clonal diversification and analysis by cell fusion. Cold Spring Harbor Symp. Quant. Biol.41, 793.
Neuberger MS & Rajewsky K (1981) Switch from hapten specific immunoglobulin M to immunoglobulin D secretion in a hybrid mouse cell line. Proc. Nat. Acad. Sci. USA78, 1138.
Radbruch A, Liesegang B, & Rajewsky K (1980) Isolation of variants of mouse myeloma X63 that express changed immunoglobulin class. Proc. Nat. Acad. Sci. USA77, 2909.
Sablitzky F, Radbruch A & Rajewsky K (1982) Spontaneous immunoglobulin class switching in myeloma and hybridoma cell lines differs from physiological class switching. Immun. Rev.67, 59.
Krawinkel V, Zoebelein G, Brüggemann M, Radbruch A & Rajewsky K (1983) Recombination between antibody heavy chain variable-region genes: evidence for gene conversion. Proc. Nat. Acad. Sci. USA80, 4997.
Cook WD & Scharff MD (1977) Antigen-binding mutants of mouse myeloma cells. Proc. Nat. Acad. Sci. USA74, 5687.
Rudikoff S, Giusti AM, Cook HD & Scharff MD (1982) Single amino acid substitution altering antigen-binding specificity. Proc. Nat. Acad. Sci. USA79, 1979.
Köhler G & Shulman M (1980) Immunoglobulin M mutants. Eur. J. Immun.10, 467.
Köhler G, Potash MJ, Lehrach H, & Shulman M (1982) Deletions in immunoglobulin mu chains. EMBO J.1, 555.
Hawley RG, Shulman JM, Murialdo H, Gibson DM & Hozumi N (1982) Mutant immunoglobulin genes have repetitive DNA elements inserted into their intervening sequences. Proc. Nat. Acad. Sci. USA79, 7425.
Sharon J, Gefter ML, Mauser T, Morrison SL, Oi VT & Ptashne M (1984) Expression of a VH-Ck chimaeric protein in mouse myeloma cells. Nature309, 364.
Köhler G, Baumann B, Iglesias A, McCubrey J, Potash MJ, Traunecker A & Zhu D (1984) Different ways to modify monoclonal antibodies. Med. Oncol & Tumor Pharmacother.1, 227.
Boulianne GL, Hozumi N & Shulman M (1984) Production of functional chimaeric mouse/human antibody. Nature312, 643.
Morrison SL, Johnson MJ, Herzenberg LA & Oi V (1984) Chimeric human antibody molecules: Mouse antigen binding domains with human constant region domains. Proc. Nat. Acad. Sci. USA81, 6851.
Neuberger MS, Williams GT & Fox RO (1984) Recombinant antibodies possessing novel effector functions. Nature312, 604.
Cobbold SP, Waldmann H (1984) Therapeutic potential of monovalent antibodies. Nature308, 460.
Milstein C & Cuello AC (1983) Hybrid hybridomas and their use in immunohistochemistry. Nature305, 537.
Köhler G (1980) Immunoglobulin chain loss in hybridoma lines. Proc. Nat. Acad. Sci. USA77, 2197.
Baumal R, Birshtein BK, Coffino P & Scharff MD (1973) Mutations in immunoglobulin-producing mouse myeloma cells. Science182, 164.
McHugh Y, Yagi M & Koshland ME (1981) The use of J and μ-chain analysis to assign lymphoid cell lines to various stages in B cell differentiation. In: B Lymphocytes in the Immune Response: Functional, Developmental and Interactive Properties (Klinman N, Mosier D, Scher I & Vitetta E, eds), p 467, Elsevier/North Holland, N.Y.
McKearn D, Huppi K, Bell M, Staudt L, Gerhard W & Weigert M (1984) Generation of antibody diversity in the immune response of Balb/c mice to influenza virus hemagglutinin. Proc. Nat. Acad. Sci. USA81, 3180.
Sablitzky F, Wildner G & Rajewsky K (1985) Somatic mutation and clonal expansion of B cells in an antigen-driven immune response. EMBO J, in press.
de Preval C & Fougereau M (1976) Specific interaction between VH and VL regions of human monoclonal immunoglobulins J. Mol. Biol.102, 657.
Klein M, Kostan C, Kells D & Dorrington K (1979) Biochemistry18, 1473.
Zhu D, Lefkovits I & Köhler G (1984) Frequency of expressed immunoglobulin light chain genes in lipopolysaccharide-stimulated Balb/c spleen cells. J. Exp. Med.160, 971.
Baumann B, Potash MJ & Köhler G (1984) Consequences of frameshift mutations at the immunoglobulin heavy chain locus of the mouse. EMBO J, in press.
Kearney JF, Radbruch A, Liesegang B & Rajewsky K (1979) A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J. Immun.123, 1548.
Shulman MJ, Heusser C, Filkin C & Köhler G (1982) Mutations affecting the structure and function of immunoglobulin M. Molec. Cell. Biol.2, 1033.
Grosschedl R, Weaver D, Baltimore D & Constantini F (1984) Introduction of μ immunoglobulin gene into the mouse germ line. Specific expression in lymphoid cells and synthesis of functional antibody. Cell38, 647.
Brinster RL, Ritchie KA, Hammer RE, O'Brien RL, Arp B & Storb U (1983) Expression of a microinjected immunoglobulin gene in the spleen of transgenic mice. Nature306, 332.
Storb U, O'Brien RL, McMullen MD, Gollahon KA & Brinster RL (1984) High expression of cloned immunoglobulin κ gene in transgenic mice is restricted to B-lympocytes. Nature310, 238.
Ritchie KA, Brinster LR & Storb U (1984) Allelic exclusion and control of endogenous immunoglobulin gene rearrangement in transgenic mice. Nature312, 517.
Rusconi S & Köhler G (1985) A transgenic mouse line transmitting and expressing a specific pair of rearranged immunoglobulin μ and κ genes. Nature, in press.
Alt WF, Yancopoulos GD, Blackwell KT, Wood C, Thomas E, Boss M, Coffmann R, Rosenberg N, Tonegawa S & Baltimore D (1984) Ordered rearrangement of immunoglobulin heavy chain variable region segments. EMBO J.3, 1209.
Kwan S, Max E, Seidman J, Leder P & Scharff M (1981) Two kappa immunoglobulin genes are expressed in the myeloma S107. Cell26, 57.
Hozumi N, Wu G, Murialdo H, Baumal R, Mosmann T, Winberry L & Marks A (1982) Arrangement of λ light chain genes in mutant clones of the MOPC31S mouse myeloma cells. J. Immunol.129, 260.
Shulman M, Wilde DC & Köhler G (1978) A better cell line for making hybridomas secreting specific antibodies. Nature276, 269–270.
Szybalski W, Szybalska EH & Ragni G (1962) Genetic studies with human cell lines. Natn. Cancer Inst. Monogr.7, 75–89.
Littlefield JW (1964) Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants. Science145, 709.
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Köhler, G. Derivation and diversification of monoclonal antibodies. Biosci Rep 5, 533–549 (1985). https://doi.org/10.1007/BF01117066
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DOI: https://doi.org/10.1007/BF01117066