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Use of purified antipeptide sera of selected specificity for the detection of the simian virus 40 large T antigen by western blotting

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Bioscience Reports

Abstract

Western blotting was used as a powerful alternative to immunoprecipitation for the detection of the simian virus 40 (SV40) large tumor (T) antigen. After resolution by electrophoresis on a SDS-polyacrylamide gel of a [15S]methionine labeled crude extract from SV40 infected monkey kidney cells, the separated proteins were transferred electrophoretically on nitrocellulose paper. T antigen was detected on nitrocellulose strips by using for the first time, specific, purified antipeptide monoclonal antibodies directed against the N- and C-terminal portions of the molecule, and125I-labeled Protein A.

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  1. Jean Bernard Dietrich

    Present address: Institut de Biologie Moléculaire et Cellulaire du CNRS, 15 rue Descartes, 67084, STRASBOURG Cedex, France

Authors and Affiliations

  1. Institut für Immunobiologie der Albert-Ludwigs-Universität, Stefan Meier Strasse 8, Freiburg i. Br., West Germany

    Jean Bernard Dietrich

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  1. Jean Bernard Dietrich
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Dietrich, J.B. Use of purified antipeptide sera of selected specificity for the detection of the simian virus 40 large T antigen by western blotting. Biosci Rep 5, 137–142 (1985). https://doi.org/10.1007/BF01117060

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  • DOI: https://doi.org/10.1007/BF01117060

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