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Polypeptide compositions of amoebae of the cellular slime mouldDictyostelium discoideum separated by partitioning during development

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Bioscience Reports

Abstract

Amoebae of the cellular slime mouldDictyostelium discoideum at 8 h or l0 h development were separated into two populations by countercurrent distribution in a dextran-poly(ethylene glycol), two-phase system. Two-dimensional, polyacrylamide-gel electrophoresis was then used to separate the polypeptides from the populations of amoebae. The two populations of amoebae at 8 h development differed sn polypeptide composition as did the populations separated at 10 h development. This confirms that cell differentiation is initated inD. discoideum prior to g h development.

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Authors and Affiliations

  1. Department of Biochemistry, University of Sheffield, S10 2TN, Sheffield, UK

    P. T. Sharpe, R. M. Sharrard & D. J. Watts

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  1. P. T. Sharpe
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  2. R. M. Sharrard
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  3. D. J. Watts
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Sharpe, P.T., Sharrard, R.M. & Watts, D.J. Polypeptide compositions of amoebae of the cellular slime mouldDictyostelium discoideum separated by partitioning during development. Biosci Rep 5, 121–127 (1985). https://doi.org/10.1007/BF01117058

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  • DOI: https://doi.org/10.1007/BF01117058

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