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Ca2+ interaction with phospholipid bilayers studied by multifrequency phase fluorometry

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Abstract

Calcium interaction with phospholipid membranes containing phosphatidic acid is studied by multifrequency phase fluorometry, using DPH as fluorescent molecule. DPH decay is analysed by a continuous distribution of lifetimes. The results suggest an increase of membrane heterogeneity at low calcium concentrations, without changes in the polarity of the environment surrounding the probe.

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Authors and Affiliations

  1. Institute of Biochemistry, Medical School, Ancona, Italy

    G. Zolese & G. Curatola

Authors
  1. G. Zolese
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  2. G. Curatola
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Zolese, G., Curatola, G. Ca2+ interaction with phospholipid bilayers studied by multifrequency phase fluorometry. Biosci Rep 9, 497–502 (1989). https://doi.org/10.1007/BF01117053

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  • DOI: https://doi.org/10.1007/BF01117053

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