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Voltage-dependent gating properties of the channel formed byE. coli hemolysin in planar lipid membranes

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Abstract

Escherichia coli hemolysin forms cation selective, ion-permeable channels of large conductance in planar phospholipid bilayer membranes. The pore formation mechanism is voltage dependent resembling that of some colicins and of diphtheria toxin: pores open when negative voltages are applied and close with positive potentials. The pH dependence of this gating process suggests that it is mediated by a negative fixed charge present in the lumen of the pore. A simple physical model of how the channel opens and closes in response to the applied voltage is given.

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Authors and Affiliations

  1. Dipartimento di Fisica, Universita' di Trento, Povo, I-38050, Trento, Italy

    Gianfranco Menestrina & Monica Ropele

Authors
  1. Gianfranco Menestrina
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  2. Monica Ropele
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Menestrina, G., Ropele, M. Voltage-dependent gating properties of the channel formed byE. coli hemolysin in planar lipid membranes. Biosci Rep 9, 465–473 (1989). https://doi.org/10.1007/BF01117049

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  • DOI: https://doi.org/10.1007/BF01117049

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