Skip to main content
SpringerLink
Log in

Recent advances in biological imaging

  • Short Papers
  • Published:
Bioscience Reports

Abstract

Recent innovations in microscopy and digital image processing have greatly enhanced the power of biological imaging. Basic principles of several new methods in light and electron microscopy will be discussed, and examples presented of their application to cell and molecular biology.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Agard, D. A. (1984) Optical sectioning microscopy: cellular architecture in three dimensions.Ann. Rev. Biophys. Bioeng. 13:191–219.

    Google Scholar 

  • Allen, R. D. (1985) New observations on cell architecture and dynamics by video-enhanced contrast optical microscopy.Ann. Rev. Biophys. Biophys. Chem. 14:265–290.

    Google Scholar 

  • Amos, L. A., Henderson, R. and Unwin, P. N. T. (1982) Three-dimensional structure determination by electron microscopy of two-dimensional crystals.Prog. Biophys. Molec. Biol. 39:183–231.

    Google Scholar 

  • Arndt-Jovin, D. J., Robert-Nicoud, M., Kaufman, S. J. and Jovin, T. M. (1985) Fluorescence digital imaging microscopy in cell biology.Science 230:247–256.

    PubMed  Google Scholar 

  • Brisson, A. and Unwin, P. N. T. (1985) Quaternary structure of the acetylcholine receptor.Nature 315:474–477.

    PubMed  Google Scholar 

  • Crowther, R. A. and Sleytr, U. B. (1977) An analysis of the fine structure of the surface layers from two strains ofClostridia, including correction for distorted images.J. Ultrastruct. Res. 58:41–49.

    PubMed  Google Scholar 

  • De Brabander, M., Nuydens, R., Geuens, G. Moeremans, M. and De May, J. (1986) The use of submicroscopic gold particles combined with video contrast enhancement as a simple molecular probe for the living cell.Cell Motility and the Cytoskeleton 6:105–113.

    PubMed  Google Scholar 

  • Dubochet, J., Adrian, M., Chang, J.-J., Homo, J. C., Lepault, J., McDowall, A. W. and Shultz, P. (1988) Cryo-electron microscopy of vitrified specimens.Quart. Rev. Biophys. 21:129–288.

    Google Scholar 

  • Gruenbaum, Y., Hochstrasser, M., Mathog, D., Saumweber, H., Agard, D. and Sedat, J. (1984) Spatial organization of theDrosophila nucleus: a three-dimensional cytogenetic study.J. Cell Sci. Suppl. 1,Higher order structure in the nucleus, (P. R. Cork and R. A. Laskey, Eds.), pp. 223–234.

  • Henderson, R., Baldwin, J. M., Downing, K. H., Lepault, J. and Zemlin, E. (1986) Structure of purple membrane fromHalobacterium halobium: recording, measurement and evaluation of electron micrographs at 3.5 Å resolution.Ultramicroscopy 19:147–178.

    Google Scholar 

  • Hiraoka, Y., Sedat, J. W. and Agard, D. A. (1987) The use of a charge-coupled device for quantitative optical microscopy of biological structures.Science 238:36–41.

    PubMed  Google Scholar 

  • Milligan, R. A. and Flicker, P. F. (1987) Structural relationships of actin, myosin and tropomyosin revealed by cryo-electron microscopy.J. Cell. Biol. 105:29–39.

    PubMed  Google Scholar 

  • Saibil, H. R. and Hewat, E. A. (1987) Ordered transmembrane and extracellular structure in squid photoreceptor microvilli.J. Cell Biol. 105:19–28.

    PubMed  Google Scholar 

  • Saxton, W. O. and Baumeister, W. (1982) The correlation averaging of a regularly arranged bacterial cell envelope protein.J. Microsc. 127:127–138.

    PubMed  Google Scholar 

  • Shotton, D. M. (1988) Review: Video-enhanced light microscopy and its applications in cell biology.J. Cell Sci. 89:129–150.

    PubMed  Google Scholar 

  • Shotton, D. M. (1989) Review: Confocal scanning fluorescence microscopy and its application for out-of-focus blur removal, non-invasive optical sectioning and three-dimensional cellular tomography of biological specimens.J. Cell Sci., in press.

  • Toyoshima, C. and Unwin, P. N. T. (1988) Ion channel of acetylcholine receptor reconstructed from images of postsynaptic membranes.Nature 336:247–250.

    PubMed  Google Scholar 

  • Tsien, R. Y. and Poenie, M. (1986) Fluorescence ratio imaging: a new window into intracellular ionic signalling.Trends Biochem. Sci. 11:450–455.

    Google Scholar 

  • White, J. G., Amos, W. B. and Fordham, M. (1987) An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy.J. Cell Biol. 105:41–48.

    PubMed  Google Scholar 

  • White, N., Lackie, P. M. and Shotton, D. M. (1989) Imaging of immunogold labelled antigens on thymocytes by confocal microscopy.Cell. Biol. Intl. Reports in press.

Download references

Author information

Author notes

  1. Helen Saibil

    Present address: Department of Crystallography, University of London Birkbeck College, Malet St., W.C.1, London, UK

Authors and Affiliations

  1. Department of Zoology, University of Oxford, South Parks Road, Oxford, UK

    Helen Saibil & Nicholas White

Authors
  1. Helen Saibil
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nicholas White
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Saibil, H., White, N. Recent advances in biological imaging. Biosci Rep 9, 437–449 (1989). https://doi.org/10.1007/BF01117046

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01117046

Key Words

Search

Navigation